Microbial Transformation of Bioactive Compounds and Production of ortho-Dihydroxyisoflavones and Glycitein from Natural Fermented Soybean Paste
Abstract
:1. Introduction
2. Results and Discussion
2.1. Soybean Doenjang and Amounts of ortho-Dihydroxyisoflavones Due to Fermentation Period
2.2. Isolation and Analysis of Isoflavone Using Microorganism Screened from Soybean Doenjang
Compound | RT in GC (min) | M+, GC/MS TMS Derivatives | TMS Substitution Pattern |
---|---|---|---|
Daidzein | 19.5 | 398 | OH-group in position C7 (A-ring) and C4' (B-ring) |
3',4'-ortho-dihydroxyisoflavone | 24.1 | 486 | OH-group in position C7 (A-ring), C3' and C4' (B-ring) |
7,8-ortho-dihydroxyisoflavone | 25.1 | 486 | OH-group in position C7, C8 (A-ring) and C4' (B-ring) |
6,7-ortho-dihydroxyisoflavone | 26.7 | 486 | OH-group in position C6, C7 (A-ring) and C4' (B-ring) |
Glycitein | 24.7 | 428 | OH-group in position C7 (A-ring) and C4' (B-ring) |
2.3. Strain Screening Strategy from Soybean Doenjang
2.4. Biotransformation of Isoflavone Using Microorganism Screened Soybean Doenjang and Identification of Reaction Products
3. Experimental
3.1. Chemicals
3.2. HPLC, NMR, GC-MS Analysis for Isolation
3.3. Plate Assay Strategy
3.4. Cultivation and Isolation of Product from Reaction
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Roh, C. Microbial Transformation of Bioactive Compounds and Production of ortho-Dihydroxyisoflavones and Glycitein from Natural Fermented Soybean Paste. Biomolecules 2014, 4, 1093-1101. https://doi.org/10.3390/biom4041093
Roh C. Microbial Transformation of Bioactive Compounds and Production of ortho-Dihydroxyisoflavones and Glycitein from Natural Fermented Soybean Paste. Biomolecules. 2014; 4(4):1093-1101. https://doi.org/10.3390/biom4041093
Chicago/Turabian StyleRoh, Changhyun. 2014. "Microbial Transformation of Bioactive Compounds and Production of ortho-Dihydroxyisoflavones and Glycitein from Natural Fermented Soybean Paste" Biomolecules 4, no. 4: 1093-1101. https://doi.org/10.3390/biom4041093