Structure and Function of the LmbE-like Superfamily
AbstractThe LmbE-like superfamily is comprised of a series of enzymes that use a single catalytic metal ion to catalyze the hydrolysis of various substrates. These substrates are often key metabolites for eukaryotes and prokaryotes, which makes the LmbE-like enzymes important targets for drug development. Herein we review the structure and function of the LmbE-like proteins identified to date. While this is the newest superfamily of metallohydrolases, a growing number of functionally interesting proteins from this superfamily have been characterized. Available crystal structures of LmbE-like proteins reveal a Rossmann fold similar to lactate dehydrogenase, which represented a novel fold for (zinc) metallohydrolases at the time the initial structure was solved. The structural diversity of the N-acetylglucosamine containing substrates affords functional diversity for the LmbE-like enzyme superfamily. The majority of enzymes identified to date are metal-dependent deacetylases that catalyze the hydrolysis of a N-acetylglucosamine moiety on substrate using a combination of amino acid side chains and a single bound metal ion, predominantly zinc. The catalytic zinc is coordinated to proteins via His2-Asp-solvent binding site. Additionally, studies indicate that protein dynamics play important roles in regulating access to the active site and facilitating catalysis for at least two members of this protein superfamily.
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Viars, S.; Valentine, J.; Hernick, M. Structure and Function of the LmbE-like Superfamily. Biomolecules 2014, 4, 527-545.
Viars S, Valentine J, Hernick M. Structure and Function of the LmbE-like Superfamily. Biomolecules. 2014; 4(2):527-545.Chicago/Turabian Style
Viars, Shane; Valentine, Jason; Hernick, Marcy. 2014. "Structure and Function of the LmbE-like Superfamily." Biomolecules 4, no. 2: 527-545.