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Biomolecules 2012, 2(2), 228-239; doi:10.3390/biom2020228

Genetic Fingerprinting of Wheat and Its Progenitors by Mitochondrial Gene orf256

1,2,* , 1
1 Department of Biotechnology, Faculty of Science, Taif University, Taif 21974, Saudi Arabia 2 Department of Genetics, Faculty of Agriculture-Chatby, University of Alexandria, Alexandria, Egypt 3 Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Cairo, Egypt
* Author to whom correspondence should be addressed.
Received: 15 March 2012 / Revised: 29 March 2012 / Accepted: 4 April 2012 / Published: 13 April 2012
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orf256 is a wheat mitochondrial gene associated with cytoplasmic male sterility (CMS) that has different organization in various species. This study exploited the orf256 gene as a mitochondrial DNA marker to study the genetic fingerprint of Triticum and Aegilops species. PCR followed by sequencing of common parts of the orf256 gene were employed to determine the fingerprint and molecular evolution of Triticum and Aegilops species. Although many primer pairs were used, two pairs of orf256 specific primers (5:-94/C: 482, 5:253/C: 482), amplified DNA fragments of 576 bp and 230 bp respectively in all species were tested. A common 500 bp of nine species of Triticum and Aegilops were aligned and showed consistent results with that obtained from other similar chloroplast or nuclear genes. Base alignment showed that there were various numbers of base substitutions in all species compared to S. cereal (Sc) (the outgroup species). Phylogenetic relationship revealed similar locations and proximity on phylogenetic trees established using plastid and nuclear genes. The results of this study open a good route to use unknown function genes of mitochondria in studying the molecular relationships and evolution of wheat and complex plant genomes.
Keywords: wheat; Triticum; Aegilops; orf256; fingerprinting; phylogenetic; molecular evolution wheat; Triticum; Aegilops; orf256; fingerprinting; phylogenetic; molecular evolution
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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El-Shehawi, A.M.; Fahmi, A.I.; Sayed, S.M.; Elseehy, M.M. Genetic Fingerprinting of Wheat and Its Progenitors by Mitochondrial Gene orf256. Biomolecules 2012, 2, 228-239.

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