Combining Dietary Intervention with Metformin Treatment Enhances Non-Alcoholic Steatohepatitis Remission in Mice Fed a High-Fat High-Sucrose Diet
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice Study Protocol
2.2. Mice Study Design
2.3. Glucose Tolerance Test
2.4. Standard Biochemical Analyses
2.5. Histological Analysis
2.6. Lipoprotein Analyses by Nuclear Magnetic Resonance (1H-NMR) Spectroscopy
2.7. Isolation of Mature Adipocytes
2.8. Semi-Targeted Lipidomics
2.9. Statistical Analysis
3. Results
3.1. Metformin Does Not Prevent HFHSD-Associated NAFLD Development and Metabolic Derangements
3.2. NAFLD Was Associated with Alterations in Cholesterol-Related Lipids and Glycerophospholipids
3.3. Age Determined the Lipid Remodeling in Adipocytes
3.4. Combining Dietary Intervention with Metformin Treatment Promotes Hepatic Steatosis Remission
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Baiges-Gaya, G.; Rodríguez-Tomàs, E.; Castañé, H.; Jiménez-Franco, A.; Amigó, N.; Camps, J.; Joven, J. Combining Dietary Intervention with Metformin Treatment Enhances Non-Alcoholic Steatohepatitis Remission in Mice Fed a High-Fat High-Sucrose Diet. Biomolecules 2022, 12, 1787. https://doi.org/10.3390/biom12121787
Baiges-Gaya G, Rodríguez-Tomàs E, Castañé H, Jiménez-Franco A, Amigó N, Camps J, Joven J. Combining Dietary Intervention with Metformin Treatment Enhances Non-Alcoholic Steatohepatitis Remission in Mice Fed a High-Fat High-Sucrose Diet. Biomolecules. 2022; 12(12):1787. https://doi.org/10.3390/biom12121787
Chicago/Turabian StyleBaiges-Gaya, Gerard, Elisabet Rodríguez-Tomàs, Helena Castañé, Andrea Jiménez-Franco, Núria Amigó, Jordi Camps, and Jorge Joven. 2022. "Combining Dietary Intervention with Metformin Treatment Enhances Non-Alcoholic Steatohepatitis Remission in Mice Fed a High-Fat High-Sucrose Diet" Biomolecules 12, no. 12: 1787. https://doi.org/10.3390/biom12121787