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Metabolites 2017, 7(1), 10; doi:10.3390/metabo7010010

Impact of Exercise and Aging on Rat Urine and Blood Metabolome. An LC-MS Based Metabolomics Longitudinal Study

1
School of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
2
School of Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
3
School of Veterinary Medicine, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
*
Author to whom correspondence should be addressed.
Academic Editor: Per Bruheim
Received: 2 January 2017 / Revised: 13 February 2017 / Accepted: 18 February 2017 / Published: 23 February 2017
(This article belongs to the Special Issue Metabolomics 2016)
View Full-Text   |   Download PDF [2167 KB, uploaded 23 February 2017]   |  

Abstract

Aging is an inevitable condition leading to health deterioration and death. Regular physical exercise can moderate the metabolic phenotype changes of aging. However, only a small number of metabolomics-based studies provide data on the effect of exercise along with aging. Here, urine and whole blood samples from Wistar rats were analyzed in a longitudinal study to explore metabolic alterations due to exercise and aging. The study comprised three different programs of exercises, including a life-long protocol which started at the age of 5 months and ended at the age of 21 months. An acute exercise session was also evaluated. Urine and whole blood samples were collected at different time points and were analyzed by LC-MS/MS (Liquid Chromatography–tandem Mass Spectrometry). Based on their metabolic profiles, samples from trained and sedentary rats were differentiated. The impact on the metabolome was found to depend on the length of exercise period with acute exercise also showing significant changes. Metabolic alterations due to aging were equally pronounced in sedentary and trained rats in both urine and blood analyzed samples. View Full-Text
Keywords: targeted metabolomics; LC-MS; rats; urine; blood; exercise; aging targeted metabolomics; LC-MS; rats; urine; blood; exercise; aging
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MDPI and ACS Style

Deda, O.; Gika, H.G.; Taitzoglou, I.; Raikos, Ν.; Theodoridis, G. Impact of Exercise and Aging on Rat Urine and Blood Metabolome. An LC-MS Based Metabolomics Longitudinal Study. Metabolites 2017, 7, 10.

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