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Metabolites 2016, 6(4), 47; doi:10.3390/metabo6040047

Metabolomics with Nuclear Magnetic Resonance Spectroscopy in a Drosophila melanogaster Model of Surviving Sepsis

1
Clinical Research, Investigation, and Systems Modeling of Acute Illness (CRISMA) Laboratory, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, 15261 PA, USA
2
Laboratoire Chimie, Structures, Propriétés de Biomatériaux et d’Agents Thérapeutiques (CSPBAT), UMR 7244, Université Paris 13, 93000 Bobigny, France
3
Intensive Care Unit, Jean Verdier University Hospital, Paris 13 University, 93140 Bondy, France
4
Laboratoire Structure—Activité des biomolécules Normales et Pathologiques (SABNP), U1204, Université d’Evry Val d’Essonne, 91025 Evry, France
5
Unité de Biologie Intégrative des Adaptations à l’Exercice (UBIAE), EA 7362, Université d’Evry Val d’Essonne, 91025 Evry, France
6
Department of Anesthesiology, University of Pittsburgh, Pittsburgh, 15261 PA, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Vladimir V. Tolstikov
Received: 28 October 2016 / Revised: 3 December 2016 / Accepted: 13 December 2016 / Published: 21 December 2016
(This article belongs to the Special Issue Metabolomics and Its Application in Human Diseases)
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Abstract

Patients surviving sepsis demonstrate sustained inflammation, which has been associated with long-term complications. One of the main mechanisms behind sustained inflammation is a metabolic switch in parenchymal and immune cells, thus understanding metabolic alterations after sepsis may provide important insights to the pathophysiology of sepsis recovery. In this study, we explored metabolomics in a novel Drosophila melanogaster model of surviving sepsis using Nuclear Magnetic Resonance (NMR), to determine metabolite profiles. We used a model of percutaneous infection in Drosophila melanogaster to mimic sepsis. We had three experimental groups: sepsis survivors (infected with Staphylococcus aureus and treated with oral linezolid), sham (pricked with an aseptic needle), and unmanipulated (positive control). We performed metabolic measurements seven days after sepsis. We then implemented metabolites detected in NMR spectra into the MetExplore web server in order to identify the metabolic pathway alterations in sepsis surviving Drosophila. Our NMR metabolomic approach in a Drosophila model of recovery from sepsis clearly distinguished between all three groups and showed two different metabolomic signatures of inflammation. Sham flies had decreased levels of maltose, alanine, and glutamine, while their level of choline was increased. Sepsis survivors had a metabolic signature characterized by decreased glucose, maltose, tyrosine, beta-alanine, acetate, glutamine, and succinate. View Full-Text
Keywords: NMR; sepsis; survival; metabolomics NMR; sepsis; survival; metabolomics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Bakalov, V.; Amathieu, R.; Triba, M.N.; Clément, M.-J.; Reyes Uribe, L.; Le Moyec, L.; Kaynar, A.M. Metabolomics with Nuclear Magnetic Resonance Spectroscopy in a Drosophila melanogaster Model of Surviving Sepsis. Metabolites 2016, 6, 47.

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