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Metabolites 2016, 6(4), 41; doi:10.3390/metabo6040041

Staphylococcus aureus Infection Reduces Nutrition Uptake and Nucleotide Biosynthesis in a Human Airway Epithelial Cell Line

1
Institute of Biochemistry, University of Greifswald, 17487 Greifswald, Germany
2
Competence Center Functional Genomics, Junior Research Group Pathoproteomics, University of Greifswald, 17487 Greifswald, Germany
*
Author to whom correspondence should be addressed.
Academic Editors: Wolfgang Eisenreich and Adelbert Bacher
Received: 6 October 2016 / Revised: 28 October 2016 / Accepted: 2 November 2016 / Published: 9 November 2016
(This article belongs to the Special Issue Carbon Metabolism)
View Full-Text   |   Download PDF [4098 KB, uploaded 11 November 2016]   |  

Abstract

The Gram positive opportunistic human pathogen Staphylococcus aureus induces a variety of diseases including pneumonia. S. aureus is the second most isolated pathogen in cystic fibrosis patients and accounts for a large proportion of nosocomial pneumonia. Inside the lung, the human airway epithelium is the first line in defence with regard to microbial recognition and clearance as well as regulation of the immune response. The metabolic host response is, however, yet unknown. To address the question of whether the infection alters the metabolome and metabolic activity of airway epithelial cells, we used a metabolomics approach. The nutrition uptake by the human airway epithelial cell line A549 was monitored over time by proton magnetic resonance spectroscopy (1H-NMR) and the intracellular metabolic fingerprints were investigated by gas chromatography and high performance liquid chromatography (GC-MS) and (HPLC-MS). To test the metabolic activity of the host cells, glutamine analogues and labelled precursors were applied after the infection. We found that A549 cells restrict uptake of essential nutrients from the medium after S. aureus infection. Moreover, the infection led to a shutdown of the purine and pyrimidine synthesis in the A549 host cell, whereas other metabolic routes such as the hexosamine biosynthesis pathway remained active. In summary, our data show that the infection with S. aureus negatively affects growth, alters the metabolic composition and specifically impacts the de novo nucleotide biosynthesis in this human airway epithelial cell model. View Full-Text
Keywords: host cell metabolism; infection; metabolomics; Staphylococcus aureus host cell metabolism; infection; metabolomics; Staphylococcus aureus
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Gierok, P.; Harms, M.; Methling, K.; Hochgräfe, F.; Lalk, M. Staphylococcus aureus Infection Reduces Nutrition Uptake and Nucleotide Biosynthesis in a Human Airway Epithelial Cell Line. Metabolites 2016, 6, 41.

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