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Metabolites 2016, 6(2), 16; doi:10.3390/metabo6020016

Metabolic Effect of Estrogen Receptor Agonists on Breast Cancer Cells in the Presence or Absence of Carbonic Anhydrase Inhibitors

1
Department of Chemistry and Biochemistry, Université de Moncton, Moncton, NB E1A 3E9, Canada
2
Atlantic Cancer Research Institute, Moncton, NB E1C 8X3, Canada
3
National Research Council of Canada, 1200 Montreal Road, Ottawa, ON, Canada
*
Author to whom correspondence should be addressed.
Academic Editors: Claudiu T. Supuran and Natalie Serkova
Received: 9 March 2016 / Revised: 27 April 2016 / Accepted: 18 May 2016 / Published: 26 May 2016
(This article belongs to the Special Issue Cancer Metabolomics 2016)
View Full-Text   |   Download PDF [5579 KB, uploaded 26 May 2016]   |  

Abstract

Metabolic shift is one of the major hallmarks of cancer development. Estrogen receptor (ER) activity has a profound effect on breast cancer cell growth through a number of metabolic changes driven by its effect on transcription of several enzymes, including carbonic anhydrases, Stearoyl-CoA desaturase-1, and oncogenes including HER2. Thus, estrogen receptor activators can be expected to lead to the modulation of cell metabolism in estrogen receptor positive cells. In this work we have investigated the effect of 17β-estradiol, an ER activator, and ferulic acid, a carbonic anhydrase inhibitor, as well as ER activator, in the absence and in the presence of the carbonic anhydrase inhibitor acetazolamide on the metabolism of MCF7 cells and MCF7 cells, stably transfected to express HER2 (MCF7HER2). Metabolic profiles were studied using 1D and 2D metabolomic Nuclear Magnetic Resonance (NMR) experiments, combined with the identification and quantification of metabolites, and the annotation of the results in the context of biochemical pathways. Overall changes in hydrophilic metabolites were largest following treatment of MCF7 and MC7HER2 cells with 17β-estradiol. However, the carbonic anhydrase inhibitor acetazolamide had the largest effect on the profile of lipophilic metabolites. View Full-Text
Keywords: 17β-estradiol; ferulic acid; carbonic anhydrase; ER + cells; metabolomics; cancer metabolism; acetazolamide 17β-estradiol; ferulic acid; carbonic anhydrase; ER + cells; metabolomics; cancer metabolism; acetazolamide
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Belkaid, A.; Čuperlović-Culf, M.; Touaibia, M.; Ouellette, R.J.; Surette, M.E. Metabolic Effect of Estrogen Receptor Agonists on Breast Cancer Cells in the Presence or Absence of Carbonic Anhydrase Inhibitors. Metabolites 2016, 6, 16.

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