Abstract: Metabolism and signalling are tightly coupled in bacteria. Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli. Experimental data based on microarrays, signalling components and extracellular metabolites are used to estimate kinetic parameters. A newly designed strain was used that adjusts the incoming glucose flux into the system and allows a kinetic analysis. Based on the results, prediction for intracelluar metabolite concentrations over a broad range of the growth rate could be performed and compared with data from literature.
Keywords: carbohydrate uptake; Escherichia coli; network component analysis; feedforward control; parameter estimation; gene regulatory network
This is an open access article distributed under the
Creative Commons Attribution License which permits unrestricted use, distribution,
and reproduction in any medium, provided the original work is properly cited.
Export to BibTeX
MDPI and ACS Style
Kremling, A.; Goehler, A.; Jahreis, K.; Nees, M.; Auerbach, B.; Schmidt-Heck, W.; Kökpinar, Ö.; Geffers, R.; Rinas, U.; Bettenbrock, K. Analysis and Design of Stimulus Response Curves of E. coli. Metabolites 2012, 2, 844-871.
Kremling A, Goehler A, Jahreis K, Nees M, Auerbach B, Schmidt-Heck W, Kökpinar Ö, Geffers R, Rinas U, Bettenbrock K. Analysis and Design of Stimulus Response Curves of E. coli. Metabolites. 2012; 2(4):844-871.
Kremling, Andreas; Goehler, Anna; Jahreis, Knut; Nees, Markus; Auerbach, Benedikt; Schmidt-Heck, Wolfgang; Kökpinar, Öznur; Geffers, Robert; Rinas, Ursula; Bettenbrock, Katja. 2012. "Analysis and Design of Stimulus Response Curves of E. coli." Metabolites 2, no. 4: 844-871.