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Analysis and Design of Stimulus Response Curves of E. coli
Systems Biotechnology, Technische Universität München, Boltzmannstr. 15, Garching b. München, Germany
University Osnabrück, Barbarastrasse 11, Osnabrück, Germany
Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, Germany
Hans Knoell Institute, Beutenbergstrasse 11a, Jena, Germany
Helmholtz Center for Infection Research, Inhoffenstr. 7, Braunschweig, Germany
Institute of Technical Chemistry-Life Science, Leibniz University of Hannover, Callinstr. 5, Hannover, Germany
* Author to whom correspondence should be addressed.
Received: 17 October 2012; Accepted: 29 October 2012 / Published: 12 November 2012
Abstract: Metabolism and signalling are tightly coupled in bacteria. Combining several theoretical approaches, a core model is presented that describes transcriptional and allosteric control of glycolysis in Escherichia coli. Experimental data based on microarrays, signalling components and extracellular metabolites are used to estimate kinetic parameters. A newly designed strain was used that adjusts the incoming glucose flux into the system and allows a kinetic analysis. Based on the results, prediction for intracelluar metabolite concentrations over a broad range of the growth rate could be performed and compared with data from literature.
Keywords: carbohydrate uptake; Escherichia coli; network component analysis; feedforward control; parameter estimation; gene regulatory network
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MDPI and ACS Style
Kremling, A.; Goehler, A.; Jahreis, K.; Nees, M.; Auerbach, B.; Schmidt-Heck, W.; Kökpinar, Ö.; Geffers, R.; Rinas, U.; Bettenbrock, K. Analysis and Design of Stimulus Response Curves of E. coli. Metabolites 2012, 2, 844-871.
Kremling A, Goehler A, Jahreis K, Nees M, Auerbach B, Schmidt-Heck W, Kökpinar Ö, Geffers R, Rinas U, Bettenbrock K. Analysis and Design of Stimulus Response Curves of E. coli. Metabolites. 2012; 2(4):844-871.
Kremling, Andreas; Goehler, Anna; Jahreis, Knut; Nees, Markus; Auerbach, Benedikt; Schmidt-Heck, Wolfgang; Kökpinar, Öznur; Geffers, Robert; Rinas, Ursula; Bettenbrock, Katja. 2012. "Analysis and Design of Stimulus Response Curves of E. coli." Metabolites 2, no. 4: 844-871.