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Metabolites 2012, 2(4), 717-732; doi:10.3390/metabo2040717
Article

Influence of the RelA Activity on E. coli Metabolism by Metabolite Profiling of Glucose-Limited Chemostat Cultures

1,* , 2
, 1
 and 1
Received: 3 August 2012; in revised form: 13 September 2012 / Accepted: 28 September 2012 / Published: 12 October 2012
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Abstract: Metabolite profiling of E. coli W3110 and the isogenic DrelA mutant cells was used to characterize the RelA-dependent stringent control of metabolism under different growth conditions. Metabolic profiles were obtained by gas chromatography–mass spectrometry (GC-MS) analysis and revealed significant differences between E. coli strains grown at different conditions. Major differences between the two strains were assessed in the levels of amino acids and fatty acids and their precursor metabolites, especially when growing at the lower dilution rates, demonstrating differences in their metabolic behavior. Despite the fatty acid biosynthesis being the most affected due to the lack of the RelA activity, other metabolic pathways involving succinate, lactate and threonine were also affected. Overall, metabolite profiles indicate that under nutrient-limiting conditions the RelA-dependent stringent response may be elicited and promotes key changes in the E. coli metabolism.
Keywords: metabolomics; metabolite profiles; stringent response; relaxed phenotype metabolomics; metabolite profiles; stringent response; relaxed phenotype
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Carneiro, S.; Villas-Bôas, S.G.; Ferreira, E.C.; Rocha, I. Influence of the RelA Activity on E. coli Metabolism by Metabolite Profiling of Glucose-Limited Chemostat Cultures. Metabolites 2012, 2, 717-732.

AMA Style

Carneiro S, Villas-Bôas SG, Ferreira EC, Rocha I. Influence of the RelA Activity on E. coli Metabolism by Metabolite Profiling of Glucose-Limited Chemostat Cultures. Metabolites. 2012; 2(4):717-732.

Chicago/Turabian Style

Carneiro, Sónia; Villas-Bôas, Silas G.; Ferreira, Eugénio C.; Rocha, Isabel. 2012. "Influence of the RelA Activity on E. coli Metabolism by Metabolite Profiling of Glucose-Limited Chemostat Cultures." Metabolites 2, no. 4: 717-732.



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