Pluronic® F-68 Enhances the Nanoparticle-Cell Interaction
AbstractNowadays, the various surfactants find wide application in pharmaceutical industry. The nanoparticle preparation process by emulsion techniques essentially requires a surfactant, most commonly Pluronic® F-68 . This non-ionic tenside influences cell physiology and was tested in clinical trial for the treatment of sickle cell disease  and myocardial infarction . Out of these reasons, even residual tenside in nanoparticle preparations might influence the cells as well as their interaction with the colloidal carriers. At this, Caco-2 single cells were incubated with fluorescent polystyrene nanoparticles, in presence of increasing amounts of Pluronic® F-68 and cell-associated nanoparticles were detected by flow cytometry. Independent from incubation temperature, the cell-associated fraction of nanoparticles concurrently increased with the tenside concentration. Ongoing from micropipette aspiration experiments this effect could be attributed to an increase of membrane stiffness of Caco-2 cells in presence of Pluronic® F-68. Furthermore, the toxicity assay revealed that viability of the cells remained unaffected at any concentration of Pluronic® F-68.
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KERLETA, V.; ANDRLIK, I.; SCHNEIDER, M.; FRANKE, T.; WIRTH, M.; GABOR, F. Pluronic® F-68 Enhances the Nanoparticle-Cell Interaction. Sci. Pharm. 2009, 77, 179.
KERLETA V, ANDRLIK I, SCHNEIDER M, FRANKE T, WIRTH M, GABOR F. Pluronic® F-68 Enhances the Nanoparticle-Cell Interaction. Scientia Pharmaceutica. 2009; 77(Short Lectures (SL)):179.Chicago/Turabian Style
KERLETA, V.; ANDRLIK, I.; SCHNEIDER, M.; FRANKE, T.; WIRTH, M.; GABOR, F. 2009. "Pluronic® F-68 Enhances the Nanoparticle-Cell Interaction." Sci. Pharm. 77, Short Lectures (SL): 179.