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Scientia Pharmaceutica is published by MDPI from Volume 84 Issue 3 (2015). Articles in this Issue were published by another publisher in Open Access under a CC-BY (or CC-BY-NC-ND) licence. Articles are hosted by MDPI on mdpi.com as a courtesy and upon agreement with Austrian Pharmaceutical Society (Österreichische Pharmazeutische Gesellschaft, ÖPhG).
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Sci. Pharm. 2009, 77(Short Lectures (SL)), 179; https://doi.org/10.3797/scipharm.oephg.21.SL-12 (registering DOI)

Pluronic® F-68 Enhances the Nanoparticle-Cell Interaction

1
Dept of Pharm. Technology a. Biopharmaceutics, Univ. of Vienna, Althanstr. 14, A-1090 Vienna, Austria
2
Institute of Physics, Univ. of Augsburg, Universitätsstr. 1, D-86135 Augsburg, Germany
*
Author to whom correspondence should be addressed.
Received: 16 April 2009 / Accepted: 16 April 2009 / Published: 16 April 2009
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Abstract

Nowadays, the various surfactants find wide application in pharmaceutical industry. The nanoparticle preparation process by emulsion techniques essentially requires a surfactant, most commonly Pluronic® F-68 [1]. This non-ionic tenside influences cell physiology and was tested in clinical trial for the treatment of sickle cell disease [2] and myocardial infarction [3]. Out of these reasons, even residual tenside in nanoparticle preparations might influence the cells as well as their interaction with the colloidal carriers. At this, Caco-2 single cells were incubated with fluorescent polystyrene nanoparticles, in presence of increasing amounts of Pluronic® F-68 and cell-associated nanoparticles were detected by flow cytometry. Independent from incubation temperature, the cell-associated fraction of nanoparticles concurrently increased with the tenside concentration. Ongoing from micropipette aspiration experiments this effect could be attributed to an increase of membrane stiffness of Caco-2 cells in presence of Pluronic® F-68. Furthermore, the toxicity assay revealed that viability of the cells remained unaffected at any concentration of Pluronic® F-68.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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KERLETA, V.; ANDRLIK, I.; SCHNEIDER, M.; FRANKE, T.; WIRTH, M.; GABOR, F. Pluronic® F-68 Enhances the Nanoparticle-Cell Interaction. Sci. Pharm. 2009, 77, 179.

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