Next Article in Journal
Validation of Fluorimetric Assay of Histone Deacetylase for Inhibitors from Plant Origin
Previous Article in Journal
An Acoustically-Driven Biochip: Particle-Cell In-teractions Under Physiological Flow Conditions
Article Menu

Article Versions

Export Article

Open AccessAbstract
Sci. Pharm. 2009, 77(Short Lectures (SL)), 179; doi:10.3797/scipharm.oephg.21.SL-12 (registering DOI)

Pluronic® F-68 Enhances the Nanoparticle-Cell Interaction

1
Dept of Pharm. Technology a. Biopharmaceutics, Univ. of Vienna, Althanstr. 14, A-1090 Vienna, Austria
2
Institute of Physics, Univ. of Augsburg, Universitätsstr. 1, D-86135 Augsburg, Germany
*
Author to whom correspondence should be addressed.
Received: 16 April 2009 / Accepted: 16 April 2009 / Published: 16 April 2009
Download PDF [214 KB, uploaded 11 May 2017]

Abstract

Nowadays, the various surfactants find wide application in pharmaceutical industry. The nanoparticle preparation process by emulsion techniques essentially requires a surfactant, most commonly Pluronic® F-68 [1]. This non-ionic tenside influences cell physiology and was tested in clinical trial for the treatment of sickle cell disease [2] and myocardial infarction [3]. Out of these reasons, even residual tenside in nanoparticle preparations might influence the cells as well as their interaction with the colloidal carriers. At this, Caco-2 single cells were incubated with fluorescent polystyrene nanoparticles, in presence of increasing amounts of Pluronic® F-68 and cell-associated nanoparticles were detected by flow cytometry. Independent from incubation temperature, the cell-associated fraction of nanoparticles concurrently increased with the tenside concentration. Ongoing from micropipette aspiration experiments this effect could be attributed to an increase of membrane stiffness of Caco-2 cells in presence of Pluronic® F-68. Furthermore, the toxicity assay revealed that viability of the cells remained unaffected at any concentration of Pluronic® F-68.
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

KERLETA, V.; ANDRLIK, I.; SCHNEIDER, M.; FRANKE, T.; WIRTH, M.; GABOR, F. Pluronic® F-68 Enhances the Nanoparticle-Cell Interaction. Sci. Pharm. 2009, 77, 179.

Show more citation formats Show less citations formats

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sci. Pharm. EISSN 2218-0532 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top