Next Article in Journal
Mouth Dissolving Tablets I: An Overview of Formulation Technology
Previous Article in Journal
Pharmacokinetic Evaluation of Wheat Germ Agglutinin-Grafted Nanoparticles of Mometasone Furoate
Article Menu

Article Versions

Export Article

Open AccessArticle
Sci. Pharm. 2009, 77(1), 133-142; doi:10.3797/scipharm.0810-07

Viability of the Human Adenocarcinoma Cell Line Caco-2: Influence of Cryoprotectant, Freezing Rate, and Storage Temperature

1
Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstraße 14, 1090, Vienna, Austria
2
ITQB-UNL/IBET, Apartado 12, 27801-901, Oeiras, Portugal
*
Author to whom correspondence should be addressed.
Received: 10 October 2008 / Accepted: 8 January 2009 / Published: 9 January 2009
Download PDF [1893 KB, uploaded 20 December 2016]

Abstract

The freezing process as well as cryostorage may induce molecular and cellular changes due to osmotic stress. Currently, standard protocols for cryopreservation of mammalian cells recommend slow freezing and rapid thawing to avoid intracellular ice crystal formation and osmotic damage. As an appropriate freezing process should guarantee adequate cell viability, we evaluated the impact of two cryoprotectants, a commercially available cryopreservation medium, two freezing rates, as well as two storage temperatures on the viability of Caco-2 cells. The freezing parameters were optimized by carefully determining vitality, cell count, proliferation, and functional differentiation of the cells. Though at least 90% of the cells were viable after one freezing/thawing cycle, adequate recovery of proliferation and differentiation is obtained not until 10 days post thawing. Interestingly, addition of 10% PEG 200 as cryoprotectant yielded best results. All in all, our results should give valuable advice for the optimization of the cryopreservation protocol of individual cell lines.
Keywords: Caco-2; Cryopreservation; Freezing rate; Cryoprotectant; Viability Caco-2; Cryopreservation; Freezing rate; Cryoprotectant; Viability
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

GÜLL, I.; MARQUES ALVES, P.; GABOR, F.; WIRTH, M. Viability of the Human Adenocarcinoma Cell Line Caco-2: Influence of Cryoprotectant, Freezing Rate, and Storage Temperature. Sci. Pharm. 2009, 77, 133-142.

Show more citation formats Show less citations formats

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Sci. Pharm. EISSN 2218-0532 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top