Next Article in Journal
The Complex Nature of Adherence in the Management of HIV/AIDS as a Chronic Medical Condition
Article Menu

Export Article

Open AccessArticle
Diseases 2013, 1(1), 1-17; doi:10.3390/diseases1010001

Protein Kinase C Epsilon Contributes to NADPH Oxidase Activation in a Pre-Eclampsia Lymphoblast Cell Model

Pharmacology and Therapeutics Group, Department of Cardiovascular Sciences, Leicester Royal Infirmary and NIHR Cardiovascular Biomedical Research Unit, Clinical Sciences Building, Leicester, LE2 7LX, UK
Current address: Endocrinology and Diabetes, Institute of Human Development, AV Hill Building University of Manchester, Manchester, M13 9PT, UK.
Author to whom correspondence should be addressed.
Received: 15 July 2013 / Revised: 16 August 2013 / Accepted: 19 August 2013 / Published: 28 August 2013
View Full-Text   |   Download PDF [1004 KB, uploaded 28 August 2013]   |  


Pre-eclampsia is a pregnancy-specific disorder characterised by hypertension and proteinuria, which in severe cases results in multi-system disturbances. The maternal syndrome is associated with a pro-inflammatory state, consisting of leukocyte activation, which is thought to contribute to the widespread endothelial dysfunction. We previously showed increased activation of NADPH oxidase in pre-eclampsia, in both neutrophils and B-lymphoblast cell lines (B-LCLs). In this study, the mechanism by which NADPH oxidase activity is increased in pre-eclampsia was further investigated. NADPH oxidase activity was found to be increased in phorbol-12-myristate-13-acetate (PMA) stimulated B-LCLs isolated from women with pre-eclampsia. This correlated with an increase in protein kinase C (PKC) substrate phosphorylation, p47-phox phosphorylation (a regulatory component of NADPH oxidase) and p47-phox directed-kinase activity. Using ion exchange and hydroxyapatite chromatography we identified a major peak of PMA regulated p47-phox kinase activity. Chromatography fractions were probed for PKC isoforms. We found the major peak of p47-phox kinase activity could not be separated from the elution profile of PKC epsilon. Using a peptide inhibitor of PKC epsilon, PMA-induced reactive oxygen species (ROS) production could be reduced to that of a normal B-LCL. These data suggest a pro-inflammatory role for PKC epsilon in the pathogenesis of pre-eclampsia. View Full-Text
Keywords: pre-eclampsia; NADPH oxidase; protein kinase C; free radicals; hypertension pre-eclampsia; NADPH oxidase; protein kinase C; free radicals; hypertension

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Poolman, T.M.; Quinn, P.A.; Ng, L. Protein Kinase C Epsilon Contributes to NADPH Oxidase Activation in a Pre-Eclampsia Lymphoblast Cell Model. Diseases 2013, 1, 1-17.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Diseases EISSN 2079-9721 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top