Optimization of Cyanine Dye Stability and Analysis of FRET Interaction on DNA Microarrays
AbstractThe application of DNA microarrays for high throughput analysis of genetic regulation is often limited by the fluorophores used as markers. The implementation of multi-scan techniques is limited by the fluorophores’ susceptibility to photobleaching when exposed to the scanner laser light. This paper presents combined mechanical and chemical strategies which enhance the photostability of cyanine 3 and cyanine 5 as part of solid state DNA microarrays. These strategies are based on scanning the microarrays while the hybridized DNA is still in an aqueous solution with the presence of a reductive/oxidative system (ROXS). Furthermore, the experimental setup allows for the analysis and eventual normalization of Förster-resonance-energy-transfer (FRET) interaction of cyanine-3/cyanine-5 dye combinations on the microarray. These findings constitute a step towards standardization of microarray experiments and analysis and may help to increase the comparability of microarray experiment results between labs. View Full-Text
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von der Haar, M.; Heuer, C.; Pähler, M.; von der Haar, K.; Lindner, P.; Scheper, T.; Stahl, F. Optimization of Cyanine Dye Stability and Analysis of FRET Interaction on DNA Microarrays. Biology 2016, 5, 47.
von der Haar M, Heuer C, Pähler M, von der Haar K, Lindner P, Scheper T, Stahl F. Optimization of Cyanine Dye Stability and Analysis of FRET Interaction on DNA Microarrays. Biology. 2016; 5(4):47.Chicago/Turabian Style
von der Haar, Marcel; Heuer, Christopher; Pähler, Martin; von der Haar, Kathrin; Lindner, Patrick; Scheper, Thomas; Stahl, Frank. 2016. "Optimization of Cyanine Dye Stability and Analysis of FRET Interaction on DNA Microarrays." Biology 5, no. 4: 47.
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