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Biology 2016, 5(2), 14; doi:10.3390/biology5020014

Differentiation of Crohn’s Disease-Associated Isolates from Other Pathogenic Escherichia coli by Fimbrial Adhesion under Shear Force

1
Institute of Electronics, Microelectronics and Nanotechnology (IEMN), UMR 8520 CNRS, Université Lille 1, Avenue Poincaré-BP 60069, 59652 Villeneuve d’Ascq, France
2
Unité de Glycobiologie Structurale et Fonctionnelle (UGSF), UMR 8576 CNRS, Université de Lille, 59000 Lille, France
3
Department of Cytology, Histology, Embryology, Danylo Halytsky Lviv National Medical University, Pekarska Str 69, 79010 Lviv, Ukraine
4
Chimie Et Interdisciplinarité, Synthèse, Analyse, Modélisation (CEISAM), UMR 6230 CNRS, LUNAM Université, 44322 Nantes Cedex 3, France
5
M2iSH, Microbes, Intestin, Inflammation and Susceptibility of the Host , UMR1071 Inserm/Université d’Auvergne, USC INRA 2018, 63000 Clermont-Ferrand, France
6
SENSIA SL, Poligono Aranguren, 9, Apdo, Correos 171, 20180 Oiartzun, Spain
7
Glycochimie des Antimicrobiens et des Agroressources, UMR 7378 CNRS, Université de Picardie Jules Verne, 80000 Amiens, France
8
Graphenea S.A., Tolosa Hiribidea, 76, E-20018 Donostia, Spain
*
Authors to whom correspondence should be addressed.
Academic Editor: Jukka Finne
Received: 28 January 2016 / Revised: 22 March 2016 / Accepted: 24 March 2016 / Published: 1 April 2016
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Abstract

Shear force exerted on uropathogenic Escherichia coli adhering to surfaces makes type-1 fimbriae stretch out like springs to catch on to mannosidic receptors. This mechanism is initiated by a disruption of the quaternary interactions between the lectin and the pilin of the two-domain FimH adhesin and transduces allosterically to the mannose-binding pocket of FimH to increase its affinity. Mannose-specific adhesion of 14 E. coli pathovars was measured under flow, using surface plasmon resonance detection on functionalized graphene-coated gold interfaces. Increasing the shear had important differential consequences on bacterial adhesion. Adherent-invasive E. coli, isolated from the feces and biopsies of Crohn’s disease patients, consistently changed their adhesion behavior less under shear and displayed lower SPR signals, compared to E. coli opportunistically infecting the urinary tract, intestines or loci of knee and hip prostheses. We exemplified this further with the extreme behaviors of the reference strains UTI89 and LF82. Whereas their FimA major pilins have identical sequences, FimH of LF82 E. coli is marked by the Thr158Pro mutation. Positioned in the inter-domain region known to carry hot spots of mutations in E. coli pathotypes, residue 158 is indicated to play a structural role in the allosteric regulation of type-1 fimbriae-mediated bacterial adhesion. View Full-Text
Keywords: adherent-invasive Escherichia coli; shear force; surface plasmon resonance; graphene; heptyl α-d-mannose adherent-invasive Escherichia coli; shear force; surface plasmon resonance; graphene; heptyl α-d-mannose
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Szunerits, S.; Zagorodko, O.; Cogez, V.; Dumych, T.; Chalopin, T.; Alvarez Dorta, D.; Sivignon, A.; Barnich, N.; Harduin-Lepers, A.; Larroulet, I.; Yanguas Serrano, A.; Siriwardena, A.; Pesquera, A.; Zurutuza, A.; Gouin, S.G.; Boukherroub, R.; Bouckaert, J. Differentiation of Crohn’s Disease-Associated Isolates from Other Pathogenic Escherichia coli by Fimbrial Adhesion under Shear Force. Biology 2016, 5, 14.

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