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Biology 2014, 3(2), 345-367; doi:10.3390/biology3020345
Review

Advanced Cell Culture Techniques for Cancer Drug Discovery

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Received: 24 February 2014; in revised form: 16 May 2014 / Accepted: 22 May 2014 / Published: 30 May 2014
(This article belongs to the Special Issue Screening for Biologically Active Compounds)
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Abstract: Human cancer cell lines are an integral part of drug discovery practices. However, modeling the complexity of cancer utilizing these cell lines on standard plastic substrata, does not accurately represent the tumor microenvironment. Research into developing advanced tumor cell culture models in a three-dimensional (3D) architecture that more prescisely characterizes the disease state have been undertaken by a number of laboratories around the world. These 3D cell culture models are particularly beneficial for investigating mechanistic processes and drug resistance in tumor cells. In addition, a range of molecular mechanisms deconstructed by studying cancer cells in 3D models suggest that tumor cells cultured in two-dimensional monolayer conditions do not respond to cancer therapeutics/compounds in a similar manner. Recent studies have demonstrated the potential of utilizing 3D cell culture models in drug discovery programs; however, it is evident that further research is required for the development of more complex models that incorporate the majority of the cellular and physical properties of a tumor.
Keywords: 3D culture; microenvironment; drug resistance; tumor models; high-throughput screening 3D culture; microenvironment; drug resistance; tumor models; high-throughput screening
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lovitt, C.J.; Shelper, T.B.; Avery, V.M. Advanced Cell Culture Techniques for Cancer Drug Discovery. Biology 2014, 3, 345-367.

AMA Style

Lovitt CJ, Shelper TB, Avery VM. Advanced Cell Culture Techniques for Cancer Drug Discovery. Biology. 2014; 3(2):345-367.

Chicago/Turabian Style

Lovitt, Carrie J.; Shelper, Todd B.; Avery, Vicky M. 2014. "Advanced Cell Culture Techniques for Cancer Drug Discovery." Biology 3, no. 2: 345-367.


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