Biology 2014, 3(1), 205-219; doi:10.3390/biology3010205
Article

Automated Sample Preparation Platform for Mass Spectrometry-Based Plasma Proteomics and Biomarker Discovery

Received: 12 December 2013; in revised form: 31 January 2014 / Accepted: 27 February 2014 / Published: 11 March 2014
(This article belongs to the Special Issue Advances in Proteomics Methods)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The identification of novel biomarkers from human plasma remains a critical need in order to develop and monitor drug therapies for nearly all disease areas. The discovery of novel plasma biomarkers is, however, significantly hampered by the complexity and dynamic range of proteins within plasma, as well as the inherent variability in composition from patient to patient. In addition, it is widely accepted that most soluble plasma biomarkers for diseases such as cancer will be represented by tissue leakage products, circulating in plasma at low levels. It is therefore necessary to find approaches with the prerequisite level of sensitivity in such a complex biological matrix. Strategies for fractionating the plasma proteome have been suggested, but improvements in sensitivity are often negated by the resultant process variability. Here we describe an approach using multidimensional chromatography and on-line protein derivatization, which allows for higher sensitivity, whilst minimizing the process variability. In order to evaluate this automated process fully, we demonstrate three levels of processing and compare sensitivity, throughput and reproducibility. We demonstrate that high sensitivity analysis of the human plasma proteome is possible down to the low ng/mL or even high pg/mL level with a high degree of technical reproducibility.
Keywords: automation; mass spectrometry; plasma; proteomics
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MDPI and ACS Style

Guryča, V.; Roeder, D.; Piraino, P.; Lamerz, J.; Ducret, A.; Langen, H.; Cutler, P. Automated Sample Preparation Platform for Mass Spectrometry-Based Plasma Proteomics and Biomarker Discovery. Biology 2014, 3, 205-219.

AMA Style

Guryča V, Roeder D, Piraino P, Lamerz J, Ducret A, Langen H, Cutler P. Automated Sample Preparation Platform for Mass Spectrometry-Based Plasma Proteomics and Biomarker Discovery. Biology. 2014; 3(1):205-219.

Chicago/Turabian Style

Guryča, Vilém; Roeder, Daniel; Piraino, Paolo; Lamerz, Jens; Ducret, Axel; Langen, Hanno; Cutler, Paul. 2014. "Automated Sample Preparation Platform for Mass Spectrometry-Based Plasma Proteomics and Biomarker Discovery." Biology 3, no. 1: 205-219.

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