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Biology 2012, 1(3), 658-667; doi:10.3390/biology1030658

Methods, Challenges and Potentials of Single Cell RNA-seq

The University of Warwick, School of Life Sciences, Coventry CV4 7AL, UK
Received: 24 October 2012 / Revised: 24 October 2012 / Accepted: 7 November 2012 / Published: 16 November 2012
(This article belongs to the Special Issue Next Generation Sequencing Approaches in Biology)
View Full-Text   |   Download PDF [221 KB, uploaded 16 November 2012]   |  

Abstract

RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several recent studies demonstrate its successful adaption to single cell analysis. This allows new biological insights into cell differentiation, cell-to-cell variation and gene regulation, and how these aspects depend on each other. Here, I review the current single cell RNA-seq (scRNA-seq) efforts and discuss experimental protocols, challenges and potentials.
Keywords: RNA-seq; scRNA-seq; single cell RNA-seq; scRNA-seq; single cell
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Hebenstreit, D. Methods, Challenges and Potentials of Single Cell RNA-seq. Biology 2012, 1, 658-667.

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