Next Article in Journal
Strategies to Block HIV Transcription: Focus on Small Molecule Tat Inhibitors
Next Article in Special Issue
Whole Genome Sequencing and a New Bioinformatics Platform Allow for Rapid Gene Identification in D. melanogaster EMS Screens
Previous Article in Journal
An Eco-Evolutionary Model for Demographic and Phenological Responses in Migratory Birds
Previous Article in Special Issue
The Population Genomics of Sunflowers and Genomic Determinants of Protein Evolution Revealed by RNAseq
Biology 2012, 1(3), 658-667; doi:10.3390/biology1030658

Methods, Challenges and Potentials of Single Cell RNA-seq

The University of Warwick, School of Life Sciences, Coventry CV4 7AL, UK
Received: 24 October 2012 / Revised: 24 October 2012 / Accepted: 7 November 2012 / Published: 16 November 2012
(This article belongs to the Special Issue Next Generation Sequencing Approaches in Biology)
View Full-Text   |   Download PDF [221 KB, uploaded 16 November 2012]   |   Browse Figure


RNA-sequencing (RNA-seq) has become the tool of choice for transcriptomics. Several recent studies demonstrate its successful adaption to single cell analysis. This allows new biological insights into cell differentiation, cell-to-cell variation and gene regulation, and how these aspects depend on each other. Here, I review the current single cell RNA-seq (scRNA-seq) efforts and discuss experimental protocols, challenges and potentials.
Keywords: RNA-seq; scRNA-seq; single cell RNA-seq; scRNA-seq; single cell
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Hebenstreit, D. Methods, Challenges and Potentials of Single Cell RNA-seq. Biology 2012, 1, 658-667.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Biology EISSN 2079-7737 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert