Next Article in Journal
Soil Oxidation-Reduction in Wetlands and Its Impact on Plant Functioning
Next Article in Special Issue
Free Energy Profile of APOBEC3G Protein Calculated by a Molecular Dynamics Simulation
Previous Article in Journal / Special Issue
Multi-Faceted Post-Transcriptional Functions of HIV-1 Rev
Article Menu

Export Article

Open AccessArticle
Biology 2012, 1(2), 175-195; doi:10.3390/biology1020175

HIV-1 Resistant CDK2-Knockdown Macrophage-Like Cells Generated from 293T Cell-Derived Human Induced Pluripotent Stem Cells

Center for Molecular Physiology Research, Children’s National Medical Center, Washington, DC 20010, USA
Department of Pediatrics, the George Washington University School of Medicine and Health Sciences, Washington, DC 20037, USA
Center for Sickle Cell Disease, Department of Medicine, Howard University, Washington, DC 20059, USA
Molecular Virology Section, Laboratory of Molecular Microbiology, NIAID, National Institutes of Health, Bethesda, MD 20892, USA
Department of Microbiology, Howard University, Washington, DC 20059, USA
Author to whom correspondence should be addressed.
Received: 15 May 2012 / Revised: 11 July 2012 / Accepted: 16 July 2012 / Published: 26 July 2012
(This article belongs to the Special Issue Structural and Molecular Biology of HIV)
View Full-Text   |   Download PDF [1004 KB, uploaded 26 July 2012]   |  


A major challenge in studies of human diseases involving macrophages is low yield and heterogeneity of the primary cells and limited ability of these cells for transfections and genetic manipulations. To address this issue, we developed a simple and efficient three steps method for somatic 293T cells reprogramming into monocytes and macrophage-like cells. First, 293T cells were reprogrammed into induced pluripotent stem cells (iPSCs) through a transfection-mediated expression of two factors, Oct-4 and Sox2, resulting in a high yield of iPSC. Second, the obtained iPSC were differentiated into monocytes using IL-3 and M-CSF treatment. And third, monocytes were differentiated into macrophage-like cells in the presence of M-CSF. As an example, we developed HIV-1-resistant macrophage-like cells from 293T cells with knockdown of CDK2, a factor critical for HIV-1 transcription. Our study provides a proof-of-principle approach that can be used to study the role of host cell factors in HIV-1 infection of human macrophages. View Full-Text
Keywords: HIV-1 resistant macrophage-like cells; CDK2 knockdown; iPSC HIV-1 resistant macrophage-like cells; CDK2 knockdown; iPSC

Figure 1

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Jerebtsova, M.; Kumari, N.; Xu, M.; Melo, G.B.A.; Niu, X.; Jeang, K.-T.; Nekhai, S. HIV-1 Resistant CDK2-Knockdown Macrophage-Like Cells Generated from 293T Cell-Derived Human Induced Pluripotent Stem Cells. Biology 2012, 1, 175-195.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Biology EISSN 2079-7737 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top