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Fibers 2018, 6(2), 27; https://doi.org/10.3390/fib6020027

Expression Analysis of Cell Wall-Related Genes in Cannabis sativa: The “Ins and Outs” of Hemp Stem Tissue Development

1
Environmental Research and Innovation Department, Luxembourg Institute of Science and Technology, 5 avenue des Hauts-Fourneaux, L-4362 Esch/Alzette, Luxembourg
2
Groupe de Recherche en Physiologie Végétale, Earth and Life Institute-Agronomy, Université Catholique de Louvain, 5 (Bte 7.07.13) Place Croix du Sud, 1348 Louvain-la-Neuve, Belgium
*
Author to whom correspondence should be addressed.
Received: 20 February 2018 / Revised: 31 March 2018 / Accepted: 10 April 2018 / Published: 1 May 2018
(This article belongs to the Special Issue Plant Bast Fibers)
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Abstract

Textile hemp (Cannabis sativa L.) is a multipurpose crop producing biomass with uses in e.g., the textile, biocomposite, and construction sectors. It was previously shown that the hypocotyl of hemp is useful to study the kinetics of secondary tissue development, where primary and secondary growths are temporally uncoupled. We here sought to demonstrate that the stem of adult hemp plants is an additional suitable model to study the heterogeneous lignification of the tissues and the mechanisms underlying secondary cell wall formation in bast fibres. A targeted quantitative PCR analysis carried out on a set of twenty genes involved in cell wall biosynthesis clearly showed differences in expression in the core and cortical tissues along four stem regions spanning from elongation to cell wall thickening. Genes involved in phenylpropanoid biosynthesis and secondary cell wall cellulose synthases were expressed at higher levels in core tissues at the bottom, while specific genes, notably a class III peroxidase and a gene partaking in lignan biosynthesis, were highly expressed in the cortex of elongating internodes. The two systems, the hypocotyl and the adult stem of textile hemp, are equally valid and complementary to address questions related to lignification and secondary cell wall deposition. View Full-Text
Keywords: bast fibre; cell wall; cellulose; lignan; lignin bast fibre; cell wall; cellulose; lignan; lignin
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Behr, M.; Lutts, S.; Hausman, J.-F.; Guerriero, G. Expression Analysis of Cell Wall-Related Genes in Cannabis sativa: The “Ins and Outs” of Hemp Stem Tissue Development. Fibers 2018, 6, 27.

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