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Fibers 2017, 5(1), 5; doi:10.3390/fib5010005

Mammalian Skeletal Muscle Fibres Promote Non-Muscle Stem Cells and Non-Stem Cells to Adopt Myogenic Characteristics

1
School of Biological Sciences, University of Reading, Reading RG5 4EW, UK
2
Freiburg Institute for Advanced Studies (FRIAS), University of Freiburg, Freiburg 79104, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Stephen C. Bondy
Received: 15 December 2016 / Revised: 16 January 2017 / Accepted: 17 January 2017 / Published: 23 January 2017
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Abstract

Skeletal muscle fibres are unique cells in large animals, often composed of thousands of post-mitotic nuclei. Following skeletal muscle damage, resident stem cells, called satellite cells, commit to myogenic differentiation and migrate to carry out repair. Satellite stem cells migrate on muscle fibres through amoeboid movement, which relies on dynamic cell membrane extension and retraction (blebbing). It is not known whether blebbing is due to the intrinsic properties of satellite cells, or induced by features of the myofibre surface. Here, we determined the influence of the muscle fibre matrix on two important features of muscle regeneration: the ability to migrate and to differentiate down a myogenic lineage. We show that the muscle fibre is able to induce amoeboid movement in non-muscle stem cells and non-stem cells. Secondly, we show that prolonged co-culture on myofibres caused amniotic fluid stem cells and breast cancer cells to express MyoD, a key myogenic determinant. Finally, we show that amniotic fluid stem cells co-cultured on myofibres are able to fuse and make myotubes that express Myosin Heavy Chain. View Full-Text
Keywords: stem cell; muscle; myofibre; adipose-derived stem cell; dental pulp; amniotic fluid stem cells; reprogramming; migration; amoeboid stem cell; muscle; myofibre; adipose-derived stem cell; dental pulp; amniotic fluid stem cells; reprogramming; migration; amoeboid
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Morash, T.; Collins-Hooper, H.; Mitchell, R.; Patel, K. Mammalian Skeletal Muscle Fibres Promote Non-Muscle Stem Cells and Non-Stem Cells to Adopt Myogenic Characteristics. Fibers 2017, 5, 5.

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