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Antibiotics 2018, 7(3), 68; https://doi.org/10.3390/antibiotics7030068

Microwave-Assisted Green Synthesis of Silver Nanoparticles Using Juglans regia Leaf Extract and Evaluation of Their Physico-Chemical and Antibacterial Properties

1
Faculty of Chemical Engineering, Sahand University of Technology, Tabriz 513351996, Iran
2
Research and Development Department, Najian Herbal Group, East Azarbaijan, Tabriz 5159193858, Iran
3
School of Engineering, Faculty of Science and Engineering, University of Waikato, Hamilton 3240, New Zealand
*
Authors to whom correspondence should be addressed.
Received: 4 July 2018 / Revised: 25 July 2018 / Accepted: 27 July 2018 / Published: 30 July 2018
(This article belongs to the Special Issue Silver-Based Antimicrobials)
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Abstract

Silver nanoparticles (Ag NPs) were synthesized using Juglans regia (J. regia) leaf extract, as both reducing and stabilizing agents through microwave irradiation method. The effects of a 1% (w/v) amount of leaf extract (0.1–0.9 mL) and an amount of 1 mM AgNO3 solution (15–25 mL) on the broad emission peak (λmax) and concentration of the synthesized Ag NPs solution were investigated using response surface methodology (RSM). Fourier transform infrared analysis indicated the main functional groups existing in the J. regia leaf extract. Dynamic light scattering, UV-Vis spectroscopy and transmission electron microscopy were used to characterize the synthesized Ag NPs. Fabricated Ag NPs with the mean particle size and polydispersity index and maximum concentration and zeta potential of 168 nm, 0.419, 135.16 ppm and −15.6 mV, respectively, were obtained using 0.1 mL of J. regia leaf extract and 15 mL of AgNO3. The antibacterial activity of the fabricated Ag NPs was assessed against both Gram negative (Escherichia coli) and positive (Staphylococcus aureus) bacteria and was found to possess high bactericidal effects. View Full-Text
Keywords: green synthesis; silver nanoparticles; microwave irradiation; Juglans regia; antibacterial activity green synthesis; silver nanoparticles; microwave irradiation; Juglans regia; antibacterial activity
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Eshghi, M.; Vaghari, H.; Najian, Y.; Najian, M.J.; Jafarizadeh-Malmiri, H.; Berenjian, A. Microwave-Assisted Green Synthesis of Silver Nanoparticles Using Juglans regia Leaf Extract and Evaluation of Their Physico-Chemical and Antibacterial Properties. Antibiotics 2018, 7, 68.

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