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Antibiotics 2016, 5(3), 22; doi:10.3390/antibiotics5030022

Techniques for Screening Translation Inhibitors

Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia
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Academic Editor: Claudio O. Gualerzi
Received: 28 February 2016 / Revised: 8 June 2016 / Accepted: 14 June 2016 / Published: 24 June 2016
(This article belongs to the Special Issue Inhibitors of the Translational Apparatus)
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Abstract

The machinery of translation is one of the most common targets of antibiotics. The development and screening of new antibiotics usually proceeds by testing antimicrobial activity followed by laborious studies of the mechanism of action. High-throughput methods for new antibiotic screening based on antimicrobial activity have become routine; however, identification of molecular targets is usually a challenge. Therefore, it is highly beneficial to combine primary screening with the identification of the mechanism of action. In this review, we describe a collection of methods for screening translation inhibitors, with a special emphasis on methods which can be performed in a high-throughput manner. View Full-Text
Keywords: translation; ribosome; antibiotic; high throughput screening translation; ribosome; antibiotic; high throughput screening
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MDPI and ACS Style

Osterman, I.A.; Bogdanov, A.A.; Dontsova, O.A.; Sergiev, P.V. Techniques for Screening Translation Inhibitors. Antibiotics 2016, 5, 22.

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