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Biosensors 2016, 6(3), 36; doi:10.3390/bios6030036

Flexible Molybdenum Electrodes towards Designing Affinity Based Protein Biosensors

1
Department of Bioengineering, University of Texas at Dallas, 800 W. Campbell Rd., Richardson, TX 75080, USA
2
EnLiSense LLC, 1813 Audubon Pond way, Allen, TX 75013, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Donatella Albanese and Roberto Pilloton
Received: 22 May 2016 / Revised: 1 July 2016 / Accepted: 11 July 2016 / Published: 18 July 2016
(This article belongs to the Special Issue Screen-Printed Electrodes and Sensors)
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Abstract

Molybdenum electrode based flexible biosensor on porous polyamide substrates has been fabricated and tested for its functionality as a protein affinity based biosensor. The biosensor performance was evaluated using a key cardiac biomarker; cardiac Troponin-I (cTnI). Molybdenum is a transition metal and demonstrates electrochemical behavior upon interaction with an electrolyte. We have leveraged this property of molybdenum for designing an affinity based biosensor using electrochemical impedance spectroscopy. We have evaluated the feasibility of detection of cTnI in phosphate-buffered saline (PBS) and human serum (HS) by measuring impedance changes over a frequency window from 100 mHz to 1 MHz. Increasing changes to the measured impedance was correlated to the increased dose of cTnI molecules binding to the cTnI antibody functionalized molybdenum surface. We achieved cTnI detection limit of 10 pg/mL in PBS and 1 ng/mL in HS medium. The use of flexible substrates for designing the biosensor demonstrates promise for integration with a large-scale batch manufacturing process. View Full-Text
Keywords: molybdenum; bioassay; flexible substrate; cardiac troponin-I; label-free biosensing molybdenum; bioassay; flexible substrate; cardiac troponin-I; label-free biosensing
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MDPI and ACS Style

Kamakoti, V.; Panneer Selvam, A.; Radha Shanmugam, N.; Muthukumar, S.; Prasad, S. Flexible Molybdenum Electrodes towards Designing Affinity Based Protein Biosensors. Biosensors 2016, 6, 36.

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