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Biosensors 2016, 6(3), 33; doi:10.3390/bios6030033

Impedimetric Label-Free Immunosensor on Disposable Modified Screen-Printed Electrodes for Ochratoxin A

1
Department of Industrial Engineering, University of Salerno, 84084 Fisciano SA, Italy
2
Institute for Microelectronics and Microsystems of the National Council of Research (CNR), 80131 Napoli, Italy
3
Institute of Atmospheric Pollution Research of the National Council of Research (CNR), 00015 Roma, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Jeff D. Newman
Received: 10 May 2016 / Revised: 7 June 2016 / Accepted: 23 June 2016 / Published: 30 June 2016
(This article belongs to the Special Issue Screen-Printed Electrodes and Sensors)
View Full-Text   |   Download PDF [3813 KB, uploaded 30 June 2016]   |  

Abstract

An impedimetric label-free immunosensor on disposable screen-printed carbon electrodes (SPCE) for quantitative determination of Ochratoxin A (OTA) has been developed. After modification of the SPCE surface with gold nanoparticles (AuNPs), the anti-OTA was immobilized on the working electrode through a cysteamine layer. After each coating step, the modified surfaces were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The capacitance was chosen as the best parameter that describes the reproducible change in electrical properties of the electrode surface at different OTA concentrations and it was used to investigate the analytical parameters of the developed immunosensor. Under optimized conditions, the immunosensor showed a linear relationship between 0.3 and 20 ng/mL with a low detection limit of 0.25 ng/mL, making it suitable to control OTA content in many common food products. Lastly, the immunosensor was used to measure OTA in red wine samples and the results were compared with those registered with a competitive ELISA kit. The immunosensor was sensitive to OTA lower than 2 μg/kg, which represents the lower acceptable limit of OTA established by European legislation for common food products. View Full-Text
Keywords: immunosensor; screen printed electrodes; Ochratoxin A; electrochemical impedance spectroscopy immunosensor; screen printed electrodes; Ochratoxin A; electrochemical impedance spectroscopy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Malvano, F.; Albanese, D.; Crescitelli, A.; Pilloton, R.; Esposito, E. Impedimetric Label-Free Immunosensor on Disposable Modified Screen-Printed Electrodes for Ochratoxin A. Biosensors 2016, 6, 33.

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