Next Article in Journal
Design and Characterization of a Sensorized Microfluidic Cell-Culture System with Electro-Thermal Micro-Pumps and Sensors for Cell Adhesion, Oxygen, and pH on a Glass Chip
Next Article in Special Issue
A Label-Free Impedance Immunosensor Using Screen-Printed Interdigitated Electrodes and Magnetic Nanobeads for the Detection of E. coli O157:H7
Previous Article in Journal
Optical Microfibre Based Photonic Components and Their Applications in Label-Free Biosensing
Previous Article in Special Issue
Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent Bacterial Adhesion
Article Menu

Export Article

Open AccessArticle
Biosensors 2015, 5(3), 500-512; doi:10.3390/bios5030500

SPR Biosensor Probing the Interactions between TIMP-3 and Heparin/GAGs

1
Center for Biotechnology and Interdisciplinary Studies, Department of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
2
Department of Biochemistry, College of Medicine, Konyang University, Konyang Hospital, Daejeon 302-718, Korea
3
Center for Biotechnology and Interdisciplinary Studies, Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
4
Departments of Biomedical Engineering, and Biological Sciences, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA
*
Authors to whom correspondence should be addressed.
Academic Editors: Nicole Jaffrezic-Renault and Carole Chaix
Received: 29 April 2015 / Revised: 8 July 2015 / Accepted: 10 July 2015 / Published: 23 July 2015
(This article belongs to the Special Issue Affinity Sensors)
View Full-Text   |   Download PDF [631 KB, uploaded 24 July 2015]   |  

Abstract

Tissue inhibitor of metalloproteinases-3 (TIMP-3) belongs to a family of proteins that regulate the activity of matrix metalloproteinases (MMPs), which can process various bioactive molecules such as cell surface receptors, chemokines, and cytokines. Glycosaminoglycans (GAGs) interact with a number of proteins, thereby playing an essential role in the regulation of many physiological/patho-physiological processes. Both GAGs and TIMP/MMPs play a major role in many cell biological processes, including cell proliferation, migration, differentiation, angiogenesis, apoptosis, and host defense. In this report, a heparin biosensor was used to map the interaction between TIMP-3 and heparin and other GAGs by surface plasmon resonance spectroscopy. These studies show that TIMP-3 is a heparin-binding protein with an affinity of ~59 nM. Competition surface plasmon resonance analysis indicates that the interaction between TIMP-3 and heparin is chain-length dependent, and N-sulfo and 6-O-sulfo groups (rather than the 2-O-sulfo groups) in heparin are important in the interaction of heparin with TIMP-3. Other GAGs (including chondroitin sulfate (CS) type E (CS-E)and CS type B (CS-B)demonstrated strong binding to TIMP-3, while heparan sulfate (HS), CS type A (CSA), CS type C (CSC), and CS type D (CSD) displayed only weak binding affinity. View Full-Text
Keywords: heparin; glycosaminoglycans; TIMP-3; binding; surface plasmon resonance heparin; glycosaminoglycans; TIMP-3; binding; surface plasmon resonance
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Zhang, F.; Lee, K.B.; Linhardt, R.J. SPR Biosensor Probing the Interactions between TIMP-3 and Heparin/GAGs. Biosensors 2015, 5, 500-512.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top