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Biosensors 2014, 4(3), 204-220; doi:10.3390/bios4030204
Article

An Inexpensive, Fast and Sensitive Quantitative Lateral Flow Magneto-Immunoassay for Total Prostate Specific Antigen

1
, 1
, 1
, 2
, 3
, 1,†
 and 1,†,*
1 Institute of Bio-Sensing Technology, University of the West of England, Bristol, BS16 1QY, UK 2 Bristol Urological Institute, Southmead Hospital, Bristol, BS10 5NB, UK 3 Department of Surgery, University Hospitals Bristol NHS Trust, Upper Maudlin Street, Bristol, BS2 8HW, UK These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 8 April 2014 / Revised: 9 June 2014 / Accepted: 13 June 2014 / Published: 8 July 2014
(This article belongs to the Special Issue Magnetic Biosensors)
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Abstract

We describe the detection characteristics of a device the Resonant Coil Magnetometer (RCM) to quantify paramagnetic particles (PMPs) in immunochromatographic (lateral flow) assays. Lateral flow assays were developed using PMPs for the measurement of total prostate specific antigen (PSA) in serum samples. A detection limit of 0.8 ng/mL was achieved for total PSA using the RCM and is at clinically significant concentrations. Comparison of data obtained in a pilot study from the analysis of serum samples with commercially available immunoassays shows good agreement. The development of a quantitative magneto-immunoassay in lateral flow format for total PSA suggests the potential of the RCM to operate with many immunoassay formats. The RCM has the potential to be modified to quantify multiple analytes in this format. This research shows promise for the development of an inexpensive device capable of quantifying multiple analytes at the point-of-care using a magneto-immunoassay in lateral flow format.
Keywords: paramagnetic particles; resonant coil magnetometer; prostate specific antigen paramagnetic particles; resonant coil magnetometer; prostate specific antigen
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Barnett, J.M.; Wraith, P.; Kiely, J.; Persad, R.; Hurley, K.; Hawkins, P.; Luxton, R. An Inexpensive, Fast and Sensitive Quantitative Lateral Flow Magneto-Immunoassay for Total Prostate Specific Antigen. Biosensors 2014, 4, 204-220.

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