Next Article in Journal
Application of a Nitric Oxide Sensor in Biomedicine
Previous Article in Journal
Recent Advances in Fluorescent Arylboronic Acids for Glucose Sensing
Biosensors 2013, 3(4), 419-428; doi:10.3390/bios3040419
Article

Development of a Fluorescence Resonance Energy Transfer (FRET)-Based DNA Biosensor for Detection of Synthetic Oligonucleotide of Ganoderma boninense

1,†
, 1,2,* , 1,2,†
 and 1,2,†
Received: 8 October 2013; in revised form: 18 November 2013 / Accepted: 19 November 2013 / Published: 12 December 2013
View Full-Text   |   Download PDF [323 KB, updated 19 December 2013; original version uploaded 12 December 2013]   |   Browse Figures
Abstract: An optical DNA biosensor based on fluorescence resonance energy transfer (FRET) utilizing synthesized quantum dot (QD) has been developed for the detection of specific-sequence of DNA for Ganoderma boninense, an oil palm pathogen. Modified QD that contained carboxylic groups was conjugated with a single-stranded DNA probe (ssDNA) via amide-linkage. Hybridization of the target DNA with conjugated QD-ssDNA and reporter probe labeled with Cy5 allows for the detection of related synthetic DNA sequence of Ganoderma boninense gene based on FRET signals. Detection of FRET emission before and after hybridization was confirmed through the capability of the system to produce FRET at 680 nm for hybridized sandwich with complementary target DNA. No FRET emission was observed for non-complementary system. Hybridization time, temperature and effect of different concentration of target DNA were studied in order to optimize the developed system. The developed biosensor has shown high sensitivity with detection limit of 3.55 × 10−9 M. TEM results show that the particle size of QD varies in the range between 5 to 8 nm after ligand modification and conjugation with ssDNA. This approach is capable of providing a simple, rapid and sensitive method for detection of related synthetic DNA sequence of Ganoderma boninense.
Keywords: Ganoderma boninense; DNA biosensor; quantum dot; fluorescence resonance energy transfer Ganoderma boninense; DNA biosensor; quantum dot; fluorescence resonance energy transfer
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Bakhori, N.M.; Yusof, N.A.; Abdullah, A.H.; Hussein, M.Z. Development of a Fluorescence Resonance Energy Transfer (FRET)-Based DNA Biosensor for Detection of Synthetic Oligonucleotide of Ganoderma boninense. Biosensors 2013, 3, 419-428.

AMA Style

Bakhori NM, Yusof NA, Abdullah AH, Hussein MZ. Development of a Fluorescence Resonance Energy Transfer (FRET)-Based DNA Biosensor for Detection of Synthetic Oligonucleotide of Ganoderma boninense. Biosensors. 2013; 3(4):419-428.

Chicago/Turabian Style

Bakhori, Noremylia M.; Yusof, Nor A.; Abdullah, Abdul H.; Hussein, Mohd Z. 2013. "Development of a Fluorescence Resonance Energy Transfer (FRET)-Based DNA Biosensor for Detection of Synthetic Oligonucleotide of Ganoderma boninense." Biosensors 3, no. 4: 419-428.


Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert