Biosensors 2012, 2(3), 245-254; doi:10.3390/bios2030245
Article

Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum

Mathieu Lazerges 1,2,* email, Hubert Perrot 1,2 email, Niriniony Rabehagasoa 1,2 email and Chantal Compère 3 email
1 Centre National de la Recherche Scientifique (CNRS), Unité Propre de Recherche (UPR) 15, Laboratoire Interfaces et Systèmes Electrochimiques, 4 place Jussieu, Paris 75252, France 2 Université Pierre et Marie Curie, LISE, 4 place Jussieu, Paris 75252, France 3 Institut Français de Recherche pour l’Exploitation de la Mer (IFREMER), Centre de Brest Service Interfaces et Capteurs, Z.I. pointe du diable, Plouzané 29280, France
* Author to whom correspondence should be addressed.
Received: 22 May 2012; in revised form: 18 June 2012 / Accepted: 27 June 2012 / Published: 4 July 2012
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Abstract: Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter time response and a higher hybridization efficiency.
Keywords: Alexandrium Minutum; avidin; biosensor; biotin; DNA; quartz crystal miocrobalance; self-assembled monolayer; thiol

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MDPI and ACS Style

Lazerges, M.; Perrot, H.; Rabehagasoa, N.; Compère, C. Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum. Biosensors 2012, 2, 245-254.

AMA Style

Lazerges M, Perrot H, Rabehagasoa N, Compère C. Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum. Biosensors. 2012; 2(3):245-254.

Chicago/Turabian Style

Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Compère, Chantal. 2012. "Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum." Biosensors 2, no. 3: 245-254.

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