Biosensors 2012, 2(2), 234-244; doi:10.3390/bios2020234
Article

Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases

1,* email, 2email, 3email and 1email
Received: 23 April 2012; in revised form: 18 May 2012 / Accepted: 25 May 2012 / Published: 29 May 2012
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST. A single use, disposable biosensor prototype, composed of catalytic iridium nano-particles dispersed on carbon paste, was developed to detect enzymatically-produced H2O2 in AST-mediated reactions. This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0–250 UL−1 specific activity). The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum. The measurements of AST in human serum using the biosensor compared well with those measured by standard hospital spectrophotometric assays. This Ir-C biosensor may be useful for AST measurements in the clinical environment.
Keywords: liver diseases; aspartate aminotransferase; electrochemical detection; amperometric biosensor
PDF Full-text Download PDF Full-Text [528 KB, uploaded 29 May 2012 14:49 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Hsueh, C.-J.; Wang, J.H.; Dai, L.; Liu, C.-C. Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases. Biosensors 2012, 2, 234-244.

AMA Style

Hsueh C-J, Wang JH, Dai L, Liu C-C. Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases. Biosensors. 2012; 2(2):234-244.

Chicago/Turabian Style

Hsueh, Chang-Jung; Wang, Joanne H.; Dai, Liming; Liu, Chung-Chiun. 2012. "Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases." Biosensors 2, no. 2: 234-244.

Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert