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Biosensors 2012, 2(2), 234-244; doi:10.3390/bios2020234
Article

Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases

1,* , 2
,
3
 and
1
1 Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA 2 Warren Alpert Medical School of Brown University, 222 Richmond Street, Providence, RI 02912, USA 3 Department of Macromolecular Science and Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA
* Author to whom correspondence should be addressed.
Received: 23 April 2012 / Revised: 18 May 2012 / Accepted: 25 May 2012 / Published: 29 May 2012
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Abstract

Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST. A single use, disposable biosensor prototype, composed of catalytic iridium nano-particles dispersed on carbon paste, was developed to detect enzymatically-produced H2O2 in AST-mediated reactions. This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0–250 UL−1 specific activity). The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum. The measurements of AST in human serum using the biosensor compared well with those measured by standard hospital spectrophotometric assays. This Ir-C biosensor may be useful for AST measurements in the clinical environment.
Keywords: liver diseases; aspartate aminotransferase; electrochemical detection; amperometric biosensor liver diseases; aspartate aminotransferase; electrochemical detection; amperometric biosensor
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Hsueh, C.-J.; Wang, J.H.; Dai, L.; Liu, C.-C. Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases. Biosensors 2012, 2, 234-244.

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