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Biosensors 2011, 1(4), 153-163; doi:10.3390/bios1040153
Article

ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin

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Received: 6 September 2011; in revised form: 13 October 2011 / Accepted: 25 October 2011 / Published: 27 October 2011
(This article belongs to the Special Issue Electrochemical Based Biosensors)
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Abstract: In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method. The ZnO nanorods were immobilized with penicillinase enzyme using the physical adsorption approach in combination with N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOS) as cross linking molecules. The potentiometric response of the sensor configuration revealed good linearity over a large logarithmic concentration range from 100 µM to 100 mM. During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin. A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed.
Keywords: zinc oxide nanorods; enzyme penicillinase; potentiometric nanosensors; N-5-azido-2-nitrobenzoyloxysuccinimide; nanodevices zinc oxide nanorods; enzyme penicillinase; potentiometric nanosensors; N-5-azido-2-nitrobenzoyloxysuccinimide; nanodevices
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Ibupoto, Z.H.; Ali, S.M.U.; Khun, K.; Chey, C.O.; Nur, O.; Willander, M. ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin. Biosensors 2011, 1, 153-163.

AMA Style

Ibupoto ZH, Ali SMU, Khun K, Chey CO, Nur O, Willander M. ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin. Biosensors. 2011; 1(4):153-163.

Chicago/Turabian Style

Ibupoto, Zafar Hussain; Ali, Syed Muhammad Usman; Khun, Kimleang; Chey, Chan Oeurn; Nur, Omer; Willander, Magnus. 2011. "ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin." Biosensors 1, no. 4: 153-163.


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