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ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin
Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping SE-60174, Sweden
* Author to whom correspondence should be addressed.
Received: 6 September 2011; in revised form: 13 October 2011 / Accepted: 25 October 2011 / Published: 27 October 2011
Abstract: In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method. The ZnO nanorods were immobilized with penicillinase enzyme using the physical adsorption approach in combination with N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOS) as cross linking molecules. The potentiometric response of the sensor configuration revealed good linearity over a large logarithmic concentration range from 100 µM to 100 mM. During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin. A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed.
Keywords: zinc oxide nanorods; enzyme penicillinase; potentiometric nanosensors; N-5-azido-2-nitrobenzoyloxysuccinimide; nanodevices
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MDPI and ACS Style
Ibupoto, Z.H.; Ali, S.M.U.; Khun, K.; Chey, C.O.; Nur, O.; Willander, M. ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin. Biosensors 2011, 1, 153-163.
Ibupoto ZH, Ali SMU, Khun K, Chey CO, Nur O, Willander M. ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin. Biosensors. 2011; 1(4):153-163.
Ibupoto, Zafar Hussain; Ali, Syed Muhammad Usman; Khun, Kimleang; Chey, Chan Oeurn; Nur, Omer; Willander, Magnus. 2011. "ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin." Biosensors 1, no. 4: 153-163.