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Biosensors 2011, 1(4), 153-163; doi:10.3390/bios1040153
Article

ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin

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Department of Science and Technology, Campus Norrköping, Linköping University, Norrköping SE-60174, Sweden
* Author to whom correspondence should be addressed.
Received: 6 September 2011 / Revised: 13 October 2011 / Accepted: 25 October 2011 / Published: 27 October 2011
(This article belongs to the Special Issue Electrochemical Based Biosensors)
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Abstract

In this study, we have successfully demonstrated the fabrication of a biosensor based on well aligned single-crystal zinc oxide (ZnO) nanorods which were grown on gold coated glass substrate using a low temperature aqueous chemical growth (ACG) method. The ZnO nanorods were immobilized with penicillinase enzyme using the physical adsorption approach in combination with N-5-azido-2-nitrobenzoyloxysuccinimide (ANB-NOS) as cross linking molecules. The potentiometric response of the sensor configuration revealed good linearity over a large logarithmic concentration range from 100 µM to 100 mM. During the investigations, the proposed sensor showed a good stability with high sensitivity of ~121 mV/decade for sensing of penicillin. A quick electrochemical response of less than 5 s with a good selectivity, repeatability, reproducibility and a negligible response to common interferents such as Na1+, K1+, d-glucose, l-glucose, ascorbic acid, uric acid, urea, sucrose, lactose, glycine, penicilloic acid and cephalosporins, was observed.
Keywords: zinc oxide nanorods; enzyme penicillinase; potentiometric nanosensors; N-5-azido-2-nitrobenzoyloxysuccinimide; nanodevices zinc oxide nanorods; enzyme penicillinase; potentiometric nanosensors; N-5-azido-2-nitrobenzoyloxysuccinimide; nanodevices
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
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Ibupoto, Z.H.; Ali, S.M.U.; Khun, K.; Chey, C.O.; Nur, O.; Willander, M. ZnO Nanorods Based Enzymatic Biosensor for Selective Determination of Penicillin. Biosensors 2011, 1, 153-163.

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