Next Article in Journal
Kinetic ELISA in Microfluidic Channels
Previous Article in Journal
Monitoring the Effect of Metal Ions on the Mobility of Artemia salina Nauplii
Biosensors 2011, 1(2), 46-57; doi:10.3390/bios1020046

Nonlinear Impedance of Whole Cells Near an Electrode as a Probe of Mitochondrial Activity

†,* ,
Department of Physics and Texas Center for Superconductivity, University of Houston, Houston, TX 77204, USA These authors equally contributed to this work.
* Author to whom correspondence should be addressed.
Received: 3 March 2011 / Revised: 25 March 2011 / Accepted: 7 April 2011 / Published: 11 April 2011
View Full-Text   |   Download PDF [509 KB, uploaded 11 April 2011]   |   Browse Figures


By simultaneously measuring the bulk media and electrode interface voltages of a yeast (Saccharomyces cerevisiae) suspension subjected to an AC voltage, a yeast-dependent nonlinear response was found only near the current injection electrodes. Computer simulation of yeast near a current injection electrode found an enhanced voltage drop across the yeast near the electrode due to slowed charging of the electrode interfacial capacitance. This voltage drop is sufficient to induce conformation change in membrane proteins. Disruption of the mitochondrial electron transport chain is found to significantly change the measured nonlinear current response, suggesting nonlinear impedance can be used as a non-invasive probe of cellular metabolic activity.
Keywords: nonlinear dielectric spectroscopy; bioimpedance; mitochondria; fermentation nonlinear dielectric spectroscopy; bioimpedance; mitochondria; fermentation
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Palanisami, A.; Mercier, G.T.; Fang, J.; Miller Jr., J.H. Nonlinear Impedance of Whole Cells Near an Electrode as a Probe of Mitochondrial Activity. Biosensors 2011, 1, 46-57.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert