Biosensors 2011, 1(1), 13-22; doi:10.3390/bios1010013
Article

Nonlinear Dielectric Spectroscopy as an Indirect Probe of Metabolic Activity in Thylakoid Membrane

1,* email, 1email, 1email, 2email and Jr. 1email
Received: 8 December 2010; in revised form: 29 December 2010 / Accepted: 27 January 2011 / Published: 31 January 2011
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Nonlinear dielectric spectroscopy (NDS) is a non-invasive probe of cellular metabolic activity with potential application in the development of whole-cell biosensors. However, the mechanism of NDS interaction with metabolic membrane proteins is poorly understood, partly due to the inherent complexity of single cell organisms. Here we use the light-activated electron transport chain of spinach thylakoid membrane as a model system to study how NDS interacts with metabolic activity. We find protein modification, as opposed to membrane pump activity, to be the dominant source of NDS signal change in this system. Potential mechanisms for such protein modifications include reactive oxygen species generation and light-activated phosphorylation.
Keywords: nonlinear dielectric spectroscopy; reactive oxygen species; whole cell biosensor; thylakoid
PDF Full-text Download PDF Full-Text [227 KB, Updated Version, uploaded 1 February 2011 09:37 CET]
The original version is still available [227 KB, uploaded 31 January 2011 11:09 CET]

Export to BibTeX |
EndNote


MDPI and ACS Style

Fang, J.; Palanisami, A.; Rajapakshe, K.; Widger, W.R.; Miller, J.H., Jr. Nonlinear Dielectric Spectroscopy as an Indirect Probe of Metabolic Activity in Thylakoid Membrane. Biosensors 2011, 1, 13-22.

AMA Style

Fang J, Palanisami A, Rajapakshe K, Widger WR, Miller JH, Jr. Nonlinear Dielectric Spectroscopy as an Indirect Probe of Metabolic Activity in Thylakoid Membrane. Biosensors. 2011; 1(1):13-22.

Chicago/Turabian Style

Fang, Jie; Palanisami, Akilan; Rajapakshe, Kimal; Widger, William R.; Miller, John H., Jr. 2011. "Nonlinear Dielectric Spectroscopy as an Indirect Probe of Metabolic Activity in Thylakoid Membrane." Biosensors 1, no. 1: 13-22.

Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert