Osteogenic Effect of ZnO-Mesoporous Glasses Loaded with Osteostatin
Abstract
:1. Introduction
2. Experimental
2.1. Synthesis of the MBGs as Powders and Processing into Disks
2.2. Physicochemical Characterization of Samples
2.3. In Vitro Studies
2.3.1. Adsorption and Release of Osteostatin
2.3.2. Assays in SBF
2.3.3. Ions Release from Disks
2.3.4. Culture Cell Studies
2.3.5. Statistical Analysis
3. Results and Discussion
3.1. Glass Powders Characterization
3.2. Textural Properties of MBG Disks
3.3. Uptake and Release of Osteostatin
3.4. In Vitro Bioactivity Assay
3.5. Degradability of Disks in Complete Medium
3.6. Cell Culture Studies
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample | 29Si | 31P | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Q4 | Q3 | Q2 | Q0 | Q1 | ||||||||||||
CS ppm | Area (%) | FWHM ppm | CS ppm | Area (%) | FWHM ppm | CS ppm | Area (%) | FWHM ppm | <Qn> | CS ppm | Area (%) | FWHM ppm | CS ppm | Area (%) | FWHM ppm | |
BL | −112 | 61.7 | 8.04 | −103 | 33.8 | 9.4 | −92 | 4.4 | 18.5 | 3.57 | 1.5 | 91.2 | 5.7 | −5.3 | 8.8 | 13.0 |
4ZN | −112 | 68 | 10.6 | −102 | 18.9 | 6.0 | −94 | 12.9 | 10.3 | 3.55 | 2.0 | 93.7 | 7.7 | −6.9 | 6.3 | 6.1 |
5ZN | −110 | 75.7 | 11.8 | −103 | 15.4 | 4.3 | −93.5 | 8.92 | 11.6 | 3.67 | 2.4 | 92.9 | 5.8 | −7.5 | 7.0 | 7.4 |
Powders | Disks | Disks + Osteostatin | Composition (EDX) Atomic % | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
SBET (m2/g) | VT (m3/g) | DP (nm) | SBET (m2/g) | VT (m3/g) | DP (nm) | SBET (m2/g) | VT (m3/g) | DP (nm) | SiO2 | CaO | P2O5 | ZnO | |
BL | 372 | 0.47 | 4.7 | 287 | 0.38 | 4.7 | 244 | 0.32 | 4.6 | 77.0 (80) | 6.1 (5) | 16.8 (15) | -- |
4ZN | 352 | 0.38 | 4.2 | 280 | 0.30 | 3.7 | 221 | 0.24 | 3.6 | 74.3 (77) | 7.0 (4.8) | 14.3 (14.4) | 4.2 (4) |
5ZN | 340 | 0.39 | 4.3 | 285 | 0.22 | 3.3 | 208 | 0.17 | 3.1 | 68.1 (76) | 9.2 (4.8) | 17.3 (14.3) | 5.3 (5) |
W0 (g/g) | A (%) | k1(*103) (h−1) | R | ||
---|---|---|---|---|---|
BL | 0.80 | 99.3 ± 3.3 | 37.7 ± 3 | 0.14 ± 0.04 | 0.996 |
4ZN | 0.90 | 99.7 ± 3.9 | 31.5 ± 3 | 0.13 ± 0.04 | 0.998 |
5ZN | 0.95 | 99.6 ± 3.9 | 31.5 ± 4 | 0.12 ± 0.07 | 0.996 |
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Pérez, R.; Sanchez-Salcedo, S.; Lozano, D.; Heras, C.; Esbrit, P.; Vallet-Regí, M.; Salinas, A.J. Osteogenic Effect of ZnO-Mesoporous Glasses Loaded with Osteostatin. Nanomaterials 2018, 8, 592. https://doi.org/10.3390/nano8080592
Pérez R, Sanchez-Salcedo S, Lozano D, Heras C, Esbrit P, Vallet-Regí M, Salinas AJ. Osteogenic Effect of ZnO-Mesoporous Glasses Loaded with Osteostatin. Nanomaterials. 2018; 8(8):592. https://doi.org/10.3390/nano8080592
Chicago/Turabian StylePérez, Rebeca, Sandra Sanchez-Salcedo, Daniel Lozano, Clara Heras, Pedro Esbrit, María Vallet-Regí, and Antonio J. Salinas. 2018. "Osteogenic Effect of ZnO-Mesoporous Glasses Loaded with Osteostatin" Nanomaterials 8, no. 8: 592. https://doi.org/10.3390/nano8080592