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Nanomaterials 2018, 8(7), 452; https://doi.org/10.3390/nano8070452

A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films

1
Department of Biomedical Engineering, University of California—Davis, Davis, CA 95616, USA
2
Department of Molecular Biosciences, University of California—Davis, Davis, CA 95616, USA
3
Department of Electrical and Computer Engineering, University of California—Davis, Davis, CA 95616, USA
*
Author to whom correspondence should be addressed.
Received: 14 May 2018 / Revised: 14 June 2018 / Accepted: 16 June 2018 / Published: 21 June 2018
(This article belongs to the Special Issue Nanoporous Gold and Other Related Materials)
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Abstract

Nanoporous gold (np-Au) electrode coatings have shown improved neural electrophysiological recording fidelity in vitro, in part due to reduced surface coverage by astrocytes. This reduction in astrocytic spreading has been attributed to the influence of electrode nanostructure on focal adhesion (FA) formation. This study describes the development and use of a microfluidic flow cell for imposing controllable hydrodynamic shear on astrocytes cultured on gold surfaces of different morphologies, in order to study the influence of nanostructure on astrocyte adhesion strength as a function of np-Au electrode morphology. Astrocyte detachment (a surrogate for adhesion strength) monotonically increased as feature size was reduced from planar surfaces to np-Au, demonstrating that adhesion strength is dependent on nanostructure. Putative mechanisms responsible for this nanostructure-driven detachment phenomenon are also discussed. View Full-Text
Keywords: nanostructure; cell-material interaction; nanoporous gold; adhesion strength; astrocyte; focal adhesion; microfluidic flow-cell nanostructure; cell-material interaction; nanoporous gold; adhesion strength; astrocyte; focal adhesion; microfluidic flow-cell
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Hampe, A.E.; Li, Z.; Sethi, S.; Lein, P.J.; Seker, E. A Microfluidic Platform to Study Astrocyte Adhesion on Nanoporous Gold Thin Films. Nanomaterials 2018, 8, 452.

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