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Article

Degradable and Photocatalytic Antibacterial Au-TiO2/Sodium Alginate Nanocomposite Films for Active Food Packaging

by
Siying Tang
1,
Zhe Wang
2,3,4,*,
Penghui Li
1,3,
Wan Li
1,
Chengyong Li
5,
Yi Wang
4 and
Paul K. Chu
1,*
1
Department of Physics and Department of Materials Science and Engineering, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China
2
Food Science and Processing Research Center, Shenzhen University, Shenzhen 518060, China
3
Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
4
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Kowloon, Hong Kong, China
5
Shenzhen Institute of Guangdong Ocean University, Shenzhen 518108, China
*
Authors to whom correspondence should be addressed.
Nanomaterials 2018, 8(11), 930; https://doi.org/10.3390/nano8110930
Submission received: 18 October 2018 / Revised: 7 November 2018 / Accepted: 8 November 2018 / Published: 8 November 2018
Browse Figures
Versions Notes

Abstract

:
A degradable and antibacterial sodium alginate film containing functional Au-TiO2 nanocomposites for food packaging was successfully developed. The Au-TiO2 nanocomposites are synthesized hydrothermally and mixed with the alginate solution to form the film by a casting method. The Au-TiO2 nanocomposites enable the film with excellent visible light absorption and transfer ability with the light absorption rang covering UV–visible wavelength (300–800 nm) and induce the increase of the film water contact angle from 40° to 74°, which contributes to the film shape stability. Furthermore, compared to the TiO2 nanoparticle-incorporated film, the antibacterial ability of Au-TiO2/sodium alginate composite film is improved approximately by 60% and 50% against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), respectively, in light conditions. The antibacterial property of the film arises from the increased production of reactive oxygen species (ROS) induced by the surface plasmonic resonance of Au nanoparticles. The degradable and antibacterial properties render the composite film of great application potential in food packaging industry.

Graphical Abstract

1. Introduction

Active food packaging offers new opportunities for food preservation and films derived from biopolymers have been widely used in food packaging due to their edible, renewable, and biodegradable characteristics [1,2,3,4,5]. Sodium alginate derived from brown algae is a green candidate to fabricate food packaging films because of advantages such as the long polymeric chain, easy accessibility, and nontoxicity [6]; it has widespread applications in the pharmaceutical, cosmetics, and biomedical fields [7,8,9,10].
To extend the functionality of active food packaging, nanoparticles such as metal nanoparticles (silver, gold, zinc), ZnO, TiO2 and CeO2 have been used to prepare composite functional films [11,12,13]. In particular, TiO2 nanoparticles (TiO2 NPs) have gained much attention due to their antibacterial activity, good photocatalytic performance, low cost, and environmental friendliness [14,15]. In 2014, the U. S. Food and Drug Administration (USFDA) approved the safety of TiO2 NPs use as food additives and for food contact substances after a safety assessment [16]. The antibacterial activity of TiO2 NPs mainly stems from the production of reactive oxygen species (ROS) such as hydroxyl radicals (OH•) and superoxide radical (O2−•) upon illumination with UV light with a wavelength of 380 nm or lower [17,18]. However, the wide bandgap of TiO2 NPs (3.0–3.2 eV) limits the light response in the UV range and so they cannot absorb and transfer visible light efficiently [19].
Incorporation of plasmonic nanoparticles, like gold (Au) and silver (Ag), into TiO2 nanostructures have been reported to enhance light absorption in the visible region due to strong surface plasmon resonance (SPR) excitation [19,20]. SPR excitation can also accelerate the interfacial charge transfer between Au and TiO2 NPs leading to higher photocatalytic activity compared to Au nanoparticles and pure TiO2 NPs [21,22,23]. Additionally, Au-incorporated TiO2 nanocomposites have been reported to show low toxicity, which renders approximately 90% eggs hatchability on the reproductive cycle of Mytilus galloprovincialis in an aquatic environment [24]. However, the Au nanoparticle-incorporated Au-TiO2 catalyst is not stable and can easily aggregate in liquid resulting in loss of catalytic efficiency and, therefore, it is important to immobilize Au-TiO2 nanocomposites in the solid film to prevent aggregation, maintain the catalytic efficiency, and produce the desirable photocatalytic antibacterial properties.
Herein, Au-TiO2 nanocomposite-incorporated sodium alginate films are prepared and applied to active food packaging. The antibacterial activity of the Au-TiO2 nanocomposite films against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) are evaluated and the photocatalytic activity is examined to elucidate the photocatalytic antibacterial mechanism. The optical and physical characteristics as well as degradation behavior of the Au-TiO2 nanocomposite-incorporated films are also determined.

2. Materials and Methods

2.1. Materials

Gold chloride trihydrate (HAuCl4 3H2O, 99.99%), sodium borohydride (NaBH4, 96%), cetyltrimethylammonium bromide (CTAB, 99%), hydrochloric acid (HCl, 37%), ascorbic acid (AA, 99%), and titanium (IV) tetrafluoride (TiF4, 99%) were purchased from Sigma-Aldrich (Hong Kong, China). Sodium alginate (SA) and glycerol were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All the reagents were used without further purification.

2.2. Experimental Methods

2.2.1. Synthesis of Au Nanoparticle Colloidal Solution

The Au nanoparticle colloidal solution was synthesized by the seed growth method with some modifications [25]. Briefly, the gold nanoseeds were prepared by adding 600 μL of NaBH4 (10 mM) to a solution of HAuCl4 (0.5 mM, 5 mL) and CTAB (0.2 M, 5 mL) under vigorous stirring. The nanoseeds solution was kept at 37 °C for 2 h in an incubator before use. The working solution was prepared by adding HAuCl4 (5 mM, 1.2 mL), NaOH (1 M, 50 μL) and AA (10 mM, 500 μL) to CTAB (0.2 M, 6 mL) sequentially. After the solution became colorless, 12 μL of the nanoseeds solution were added rapidly into the working solution. It was mixed gently and stored in an incubator at 37 °C overnight. The Au nanoparticles were obtained by centrifugation at 10,000 rpm for 10 min to remove unreacted reagents and washing with deionized water twice.

2.2.2. Preparation of Au-TiO2 Nanocomposites and TiO2 Nanoparticles

The Au-TiO2 nanocomposites were prepared using the procedures described in the literature with some modification [26]; 90 μL of the aqueous TiF4 solution was dropped into 10 mL of the Au nanoparticle colloidal solution under vigorous stirring. The mixture was transferred to a Teflon-lined stainless-steel autoclave and the hydrothermal reaction was conducted at 180 °C for 12 h. The solution was cooled to room temperature and centrifuged at 7000 rpm for 10 min. The Au-TiO2 nanocomposites were washed with water three times to remove the remaining reagents. The final samples were dried at 40 °C in an oven and collected for further use. For comparison, pure TiO2 NPs were prepared by low-temperature controlled hydrolysis of TiCl4 in water [27,28].

2.2.3. Film Preparation

The sodium alginate/Au-TiO2 nanocomposite (SAT) films were prepared by casting-solvent evaporation [3]. The SA powder was dissolved in distilled water at 70 °C for 30 min under magnetic stirring to form a homogenous solution (10%, w/v) and different amounts of the Au-TiO2 nanocomposite were added to the SA solution (0 and 2.5 wt%). Glycerol (10 wt% based on the content of dried matter) was added as the plasticizer [29] and the film-forming solutions were placed under vacuum for 60 min at room temperature to remove bubbles. Finally, the solutions were put on glass petri dishes and dried at 40 °C for 24 h. For comparison, the same amount of TiO2 NPs was mixed with the SA solution (2.5 wt%) to fabricate the sodium alginate/TiO2 NPs (ST) film.

2.3. Microstructure Characterization

The UV–vis absorption spectra were acquired on the UV spectrophotometer (HALO DB-20 UV–VIS Double Beam Spectrophotometer, Dynamic Company, Livingston, UK) in the 300–800 nm range. High-resolution transmission electron microscopy (HR-TEM, JEOL-2100F, Tokyo, Japan) and field-emission scanning electron microscopy (FEI NOVA NANO SEM 450, Oregon, USA) were used to identify the size and shape of the nanocomposite and morphology of the films. The stability of the particles against agglomeration was evaluated by the zeta potential measurement (Model Zeta Sizer Nano ZS, Nano-ZS90, Malvern Instruments, Malvern, UK). Generally, 0.5 mL of the Au-TiO2 nanocomposite suspension (0.5 g/L) was transferred to a folded capillary cell with two caps (DTS 1070, Malvern Instruments, Malvern, UK) and the measurements were performed at room temperature in triplicates.

2.4. Water Contact Angle Test

A contact angle meter (OCA20, Dataphysics Co., Ltd., Filderstadt, Germany) was used to measure the surface contact angle. The sample (10 × 50 mm) was kept on a movable sample stage and leveled horizontally. 2 μL of distilled water was placed on the film using a micro-syringe and the contact angle was measured three times.

2.5. Photocatalytic Activity of the Sodium Alginate/Au-TiO2 Nanocomposite Film

The SAT film was dissolved in boiling water under stirring for 6 h to acquire the Au-TiO2 nanocomposite solution. The 0.5 mg/mL Au-TiO2 nanocomposite solution (10 mL) was dispersed in 20 mL of a solution containing methanol, ethanol, and 0.4 mM 5,5-dimethyl-1-pyrroline N-oxide (DMPO) frequently used for free radical trapping. The solution was stirred continuously in darkness for about 1 h to establish the absorption-desorption equilibrium and ESR spectra (JEOL FA100 Spectrometer, Eching b. München, Germany) were collected from the solution containing DMPO and nanoparticles (TiO2 NPs or Au-TiO2 nanocomposites) after exposure to light (λ > 380 nm) for a selected period of time.

2.6. Antibacterial Evaluation

The antibacterial activity of the SAT films (round film with diameter of 10 cm) was probed by examining their inhibitory effects on the growth of E. coli and S. enterica under light and dark conditions. After sequential ten-fold dilution with normal saline, the bacterial suspensions with 105 and 106 CFU/mL were obtained. The same amounts of bacteria (0.1 mL) were used for the Au-TiO2 nanocomposites and TiO2 NPs contained sodium alginate solution (10 mL). The light was generated from a 10 W LED and the exposure time was 20 min. The bacteria survival rate was calculated by the following equation: Survival rate (%) = N/N0 × 100, where N is the number of colonies forming units counted in the presence of Au-TiO2 nanocomposites or TiO2 NPs and N0 is the number of colonies forming units in presence of saline solution.

2.7. Degradation Property

The degradation property of the film was studied by measuring the kinematic viscosity reduction of the film solution. The SA solutions with and without NPs (TiO2 NPs or Au-TiO2 nanocomposites) were exposed to one sun (ABET Sun 2000 simulator, class A, Milford, CT, USA) for 8 h each day for 35 days. The kinematic viscosity tests were performed on an automatic viscometer at 25 °C (DV–I Digital Viscometer, Brookfield Ltd., Middleboro, MA, USA) using 25 mL of the film solutions obtained on different days.

2.8. Statistical Analysis

All the samples were analyzed in triplicate and the one-way analysis of variance (ANOVA) was applied to the data followed by Duncan to distinguish the treatments at p < 0.05. The statistical analyses were performed using the SPSS version 17.0 (SPSS Inc., Chicago, IL, USA).

3. Results and Discussion

3.1. Properties of Au NPs, TiO2 NPs, and Au-TiO2 Nanocomposites

Figure 1a shows the UV–vis absorption spectra of the Au NPs, TiO2 NPs and Au-TiO2 nanocomposites films. Light absorption by TiO2 NPs occurs mainly in the UV region below 380 nm, whereas that by the Au NPs is in the visible range (400–600 nm) with a peak at 512 nm. In comparison, the Au-TiO2 nanocomposites exhibit broad absorption from 300 to 800 nm, possibly because the valence band electrons in the TiO2 shell are excited at wavelengths longer than 380 nm with the aid of the Au core triggered localized energy level in the bandgap of TiO2 [30]. Owing to plasmonic absorption by the Au core in the Au-TiO2 nanocomposites, an additional absorption band (500–800 nm) is observed in the visible region. The increased visible light absorption is affected mostly by incorporation of the Au core, thereby indicating the positive effect on the visible light photocatalytic activity [31].
The morphology of the nanoparticles is observed by TEM. The Au NPs possess a spherical shape with a diameter of about 45 nm (Figure 1b), whereas the pure TiO2 NPs have a popcorn shape but poor dispersing properties (Figure 1c). The aggregation tendency of TiO2 NPs stems from the porous structure and surface charge. During synthesis, a large number of protons are released producing positive charges on the TiO2 NPs (Figure S1) [28]. Moreover, TiO2 NPs have a large surface area and high surface energy, and since no capping agents are attached to the TiO2 NPs surface, they are likely to aggregate and disperse poorly. However, during the synthesis of the Au NPs, positively charged CTAB molecules are the capping agents for the Au NPs forming a bilayer template close to the surface. CTAB is a surfactant soluble in both water and oil and the extra charges on the CTAB-coated Au NPs produce a repulsive force among the Au nanoparticles and improves the dispersity in water. Figure 1d shows the core-shell structure of the Au-TiO2 nanocomposites with TiO2 NPs surrounding the Au NPs core forming the TiO2 shell. Figure 1e reveals the interplanar spacings of the materials with Au (1,1,1) being 2.35 Å and anatase TiO2 (1,0,1) being 3.5 Å and Figure 1f confirms the elemental components in the Au-TiO2 nanocomposites [32].

3.2. Film Preparation and Characterization

Figure 2a illustrates the fabrication process of the degradable SA/Au-TiO2 nanocomposite (SAT) film. The SAT film has a purple semi-transparent appearance arising from the Au-TiO2 nanocomposite but the SA films are transparent. For SAT films designed for packaging, transparency is an important factor. Light can go through transparent films to increase heat and photo-induced ROS affecting the contents. In comparison, the colored SAT film can block light to prevent or slow putridness and photodegradation of photosensitive nutrient contents, such as vitamin C, vitamin D and some proteins. As shown in the SEM images in Figure 2b,c, both the SA and SAT films have a smooth and continuous surface and the SAT film contains evenly distributed Au-TiO2 nanocomposites. The Au-TiO2 nanocomposites are randomly distributed on the film or imbedded in the film without destroying the film continuity (Figure 2d).
Water sensitivity plays an important role in food packaging and the water contact angle reflects the water sensitivity. The SA films are kept at room temperature in a moist environment for one day and the photos in Figure 3a are taken after drying. Both the ST film and SAT film maintain a good shape and morphology, but the shape of the pure alginate film changes due to the reaction with water in air. The contact angle on the pure alginate film is 40° (Figure 3b) implying that the film has good affinity to water and the shape can change easily due to moisture absorption. The poor water resistance of the pure alginate film is due to the hydrophilic nature of alginate and the small amount of glycerol (plasticizer) added to enhance the film flexibility. When a certain amount of TiO2 NPs is added, the contact angle on the film increases to 53° (Figure 3c) and less water adsorbs onto the film. By adding the same amount of Au-TiO2 nanocomposites, the contact angle of the film increases further to 74° (Figure 3d). The film is less likely to be affected by shape changes caused by moisture, thus, boding well for food packaging. The improved water resistance also arises from the morphological change after addition of nanoparticles [33,34]. The results indicate that adding Au-TiO2 nanocomposites prevents the spreading of water drops on the film and increases the surface hydrophobicity.

3.3. Antibacterial Activity and Photocatalytic Antibacterial Mechanism

As shown in Figure 4, both the SAT and ST films have good antibacterial properties against S. aureus and E. coli in the absence of light. With regard to the SAT film, the survival rates of S. aureus and E. coli are 10% and 12.9%, respectively and small differences are observed from the ST film. However, in the presence of light, the antibacterial properties of SAT against S. aureus and E. coli improve significantly but those of the ST film are enhances only slightly. The SAT film shows significantly higher (p < 0.05) antimicrobial efficacy than the SA film, suggesting that the Au-TiO2 nanocomposites have enhanced light absorption and transfer ability due to the plasmonic effect. Compared to TiO2 nanoparticles, incorporated nanocellulose films in the literature, the developed film presents much higher antibacterial effectiveness [35], which may be due to the SPR effect of the Au core in Au-TiO2 nanocomposite.
The photocatalytic activity of the SAT film and resulting ROS production are believed to be the main mechanisms for the enhanced antibacterial characteristics under light irradiation. Light can activate the Au-TiO2 nanocomposites to produce ROS when the film is used as a packaging material with light from outside the package. Here, the photocatalytic activity is studied and ERS spectra are recorded from samples containing Au-TiO2 nanocomposites and spin trap (DMPO) with and without visible light (>380 nm) irradiation are shown in Figure 5. No ESR signal is observed from DMPO in the dark environment but after irradiation with visible light for 10 min, a four-line spectrum with relative intensities of 1:2:2:1 from OH• in the aqueous dispersion of DMPO is observed (Figure 5a) [33]. As shown in Figure 5d, a longer exposure time to visible light leads to increased intensity, which can be directly correlated with the abundance of DMPO-OH. Meanwhile, the maximum intensity is observed after an exposure time of 10 min and the spectrum acquired after exposure for 20 min is less intense that that after 10 min. Therefore, 10 min is the optimal exposure time and adopted in the subsequent study. O2−•/HO2• radicals are detected from methanol and ethanol and have smaller intensities in the characteristic signal of 1:1:1:1 (Figure 5b,c). Similarly, there is no signal under dark conditions, showing that visible light triggers the generation of radicals on the Au-TiO2 nanocomposites. For comparison, no ROS signals are observed under both dark and light conditions from the TiO2 NPs, indicating that the Au NPs help to harvest and transfer the photons to the Au-TiO2 nanocomposites.
Figure 5e shows that the formation of oxygen radicals under light irradiation originates from electron transfer of the semiconductor. When a semiconductor is irradiated with light with energy no less than the bandgap, electrons on the valence band can be excited to the conduction band to create charge carries, namely holes and electrons. The holes and electrons possess strong oxidative and reductive ability, respectively, leading to easy formation of ROS in aqueous media. However, TiO2 nanoparticles have a bandgap of 3.2 eV at a pH of 7 and so absorb UV light. Owing to the contact with the Au nanostructure, separation of electrons and holes and generation of ROS under visible light irradiation become easier and more efficient. Firstly, Au has a lower Fermi level compared to EC (conductive energy level) of TiO2 and therefore, it can contribute to the capture of electrons from the TiO2 conduction band and enhance separation of electrons and holes to produce ROS. Secondly, visible light can produce the SPR effect on the Au nanoparticles resulting in hot electrons or local electrical field enhancement. Accordingly, both hot electrons and local electrical field enhancement enhance production of ROS [25].
Based on our results, the main factors responsible for the antibacterial properties of the Au-TiO2-incorporated composite films are as follows. First of all, incorporation of Au-TiO2 nanocomposites enhances the surface hydrophobicity of the films. Secondly, the nanostructure increases the contact area with microorganisms. Thirdly, photoexcitation of electrons in TiO2 NPs scarcely occurs, but the Au core is aided by visible light absorption in the Au-TiO2 nanocomposite and the SPR effect contributes to electron transfer from the valence state to conduction state of TiO2. The ROS produced enhance the antibacterial efficiency of the SAT films compared to the ST films.

3.4. Degradation Properties of Au-TiO2/Sodium Alginate Composites

According to the Mark–Houwink equation, the average molecules in sodium alginate exhibit a positive correlation with the solution viscosity at a certain concentration [36]. Figure 6 shows that the pure alginate solution degrades very fast in the beginning, but degradation slows 15 days later. In comparison, the alginate solution with Au-TiO2 nanocomposites degrades slowly initially due to poor water adsorption. Moreover, the viscosity of the alginate solution with the Au-TiO2 nanocomposite is lower than that of the pure alginate solution after exposure to sunlight for 35 days, indicating that residuals in the alginate solution with the Au-TiO2 nanocomposite have shorter chains and smaller molecular weight than those in the pure alginate solution. Therefore, the degradation effect of the alginate solution is improved by adding the Au-TiO2 nanocomposite.

4. Conclusions

A degradable alginate film containing Au-TiO2 nanocomposites is prepared. It has the ability to adsorb and transfer visible light in addition to the shape stability, antibacterial properties, and easy degradation under sunlight. The Au-TiO2 nanocomposites were synthesized hydrothermally and mixed with the alginate solution to form films by the casting method. The excellent properties can be attributed to surface plasmonic resonance of Au nanoparticles enhancing light absorption and conversion. Visible light produces free radicals on the Au-TiO2 nanocomposites giving rise to improved antibacterial properties under light irradiation, and degradation is enhanced.

Supplementary Materials

The following are available online at https://www.mdpi.com/2079-4991/8/11/930/s1, Figure S1: Zeta potentials of Au nanoparticles, TiO2 nanoparticles, and Au-TiO2 nanocomposites.

Author Contributions

Conceptualization, S.T. and Z.W.; Formal analysis, S.T. and Z.W.; Funding acquisition, Z.W., P.L. and P.K.C.; Investigation, S.T., Z.W. and W.L.; Methodology, S.T. and Z.W.; Resources, C.L. and Y.W.; Supervision, P.K.C.; Writing–original draft, S.T.; Writing–review and editing, Z.W., P.L. and P.K.C.

Funding

This work was jointly supported by Shenzhen overseas special fund for high-level talents [No. KQJSCX2017033116171850], Shenzhen Science and Technology Research Funding [No. JCYJ20160608141439330], Shenzhen Science and Technology research and development Research Project [No. JSGG20160229201849420], and Hong Kong Research Grants Council (RGC) General Research Funds (GRF) Nos. CityU 11301215 and 11205617.

Acknowledgments

We would like to thank Shenzhen Graduate School, Peking University for TEM characterization.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. (a) UV–vis absorption spectra of the Au NPs, TiO2 NPs and Au-TiO2 nanocomposites films; TEM images of (b) Au NPs, (c) TiO2 NPs, and (d–f) Au-TiO2 nanocomposites.
Figure 1. (a) UV–vis absorption spectra of the Au NPs, TiO2 NPs and Au-TiO2 nanocomposites films; TEM images of (b) Au NPs, (c) TiO2 NPs, and (d–f) Au-TiO2 nanocomposites.
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Figure 2. (a) Schematic illustration of the fabricating process of the Au-TiO2 nanocomposite film; SEM images of (b) pure alginate film and (c,d) Au-TiO2 nanocomposite film.
Figure 2. (a) Schematic illustration of the fabricating process of the Au-TiO2 nanocomposite film; SEM images of (b) pure alginate film and (c,d) Au-TiO2 nanocomposite film.
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Figure 3. (a) Photos of different films after drying process; Contact angles: (b) Alginate film, (c) TiO2 NPs alginate film, and (d) Au-TiO2 nanocomposite alginate film.
Figure 3. (a) Photos of different films after drying process; Contact angles: (b) Alginate film, (c) TiO2 NPs alginate film, and (d) Au-TiO2 nanocomposite alginate film.
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Figure 4. Antibacterial properties against S. aureus and E. coli under dark and visible light conditions.
Figure 4. Antibacterial properties against S. aureus and E. coli under dark and visible light conditions.
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Figure 5. The electron spin resonance (ERS) results of (a) OH• radical and O2• radial under (b) dark and (c) light conditions; (d) Intensity change of OH• radical for different irradiation time; (e) Mechanism of radical generation; (f) Comparison of ERS results of the TiO2 nanoparticles.
Figure 5. The electron spin resonance (ERS) results of (a) OH• radical and O2• radial under (b) dark and (c) light conditions; (d) Intensity change of OH• radical for different irradiation time; (e) Mechanism of radical generation; (f) Comparison of ERS results of the TiO2 nanoparticles.
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Figure 6. Degradation curve of the pure alginate and Au-TiO2 nanocomposites added alginate solutions.
Figure 6. Degradation curve of the pure alginate and Au-TiO2 nanocomposites added alginate solutions.
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MDPI and ACS Style

Tang, S.; Wang, Z.; Li, P.; Li, W.; Li, C.; Wang, Y.; Chu, P.K. Degradable and Photocatalytic Antibacterial Au-TiO2/Sodium Alginate Nanocomposite Films for Active Food Packaging. Nanomaterials 2018, 8, 930. https://doi.org/10.3390/nano8110930

AMA Style

Tang S, Wang Z, Li P, Li W, Li C, Wang Y, Chu PK. Degradable and Photocatalytic Antibacterial Au-TiO2/Sodium Alginate Nanocomposite Films for Active Food Packaging. Nanomaterials. 2018; 8(11):930. https://doi.org/10.3390/nano8110930

Chicago/Turabian Style

Tang, Siying, Zhe Wang, Penghui Li, Wan Li, Chengyong Li, Yi Wang, and Paul K. Chu. 2018. "Degradable and Photocatalytic Antibacterial Au-TiO2/Sodium Alginate Nanocomposite Films for Active Food Packaging" Nanomaterials 8, no. 11: 930. https://doi.org/10.3390/nano8110930

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