SERS-Based Flavonoid Detection Using Ethylenediamine-β-Cyclodextrin as a Capturing Ligand
AbstractEthylenediamine-modified β-cyclodextrin (Et-β-CD) was immobilized on aggregated silver nanoparticle (NP)-embedded silica NPs (SiO2@Ag@Et-β-CD NPs) for the effective detection of flavonoids. Silica NPs were used as the template for embedding silver NPs to create hot spots and enhance surface-enhanced Raman scattering (SERS) signals. Et-β-CD was immobilized on Ag NPs to capture flavonoids via host-guest inclusion complex formation, as indicated by enhanced ultraviolet absorption spectra. The resulting SiO2@Ag@Et-β-CD NPs were used as the SERS substrate for detecting flavonoids, such as hesperetin, naringenin, quercetin, and luteolin. In particular, luteolin was detected more strongly in the linear range 10−7 to 10−3 M than various organic molecules, namely ethylene glycol, β-estradiol, isopropyl alcohol, naphthalene, and toluene. In addition, the SERS signal for luteolin captured by the SiO2@Ag@Et-β-CD NPs remained even after repeated washing. These results indicated that the SiO2@Ag@Et-β-CD NPs can be used as a rapid, sensitive, and selective sensor for flavonoids. View Full-Text
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Description: Figure S1. Schematic illustration of the synthesis of Et-β-CD@Ag@SiO2 NPs. Figure S2. 1D NMR spectra of Et-β-CD. (a) 1H NMR spectrum; (b) 13C NMR spectrum. The NMR experiments were carried out on a Brucker Avance spectrometer at 600 MHz in a D2O solution at 25 °C. Figure S3. UV-Vis absorbance of SiO2@Ag@Et-β-CD NPs reacted with Nar, Hes, Lut, and Que. Figure S4. TEM images of Ag@SiO2 NPs in EtOH Ag@SiO2 NPs measured using an energy-filtering transmission electron microscope (EF-TEM, LIBRA 120, Carl Zeiss, Oberkochen, Germany ). The accelerating voltage was 120 kV. Figure S5. Surface-enhanced Raman scattering (SERS) spectra. (a) SERS spectra of organic molecu-les after mixing with Et-β-CD@Ag@SiO2 NPs (i. no target, ii. ethylene glycol, iii. β-estradiol, iv. Isop-ropyl alcohol, v. naphthalene, vi. toluene and vii. Lut); (b) SERS spectra of (i) SiO2@Ag NPs, (ii) SiO2@Ag NPs reacted with Aniline, (iii) SiO2@Ag@Et-β-CD reacted with Aniline, and (iv) SiO2@Ag@Et-β--CD NPs reacted with mixing solution of Lut and aniline. Figure S6. Surface-enhanced Raman scattering (SERS) spectra and normalized SERS intensity graph. SERS spectra of SiO2@ Ag NPs mixed with Lut at 1 × 10−2 M to 1 × 10−7 M.
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Choi, J.M.; Hahm, E.; Park, K.; Jeong, D.; Rho, W.-Y.; Kim, J.; Jeong, D.H.; Lee, Y.-S.; Jhang, S.H.; Chung, H.J.; Cho, E.; Yu, J.-H.; Jun, B.-H.; Jung, S. SERS-Based Flavonoid Detection Using Ethylenediamine-β-Cyclodextrin as a Capturing Ligand. Nanomaterials 2017, 7, 8.
Choi JM, Hahm E, Park K, Jeong D, Rho W-Y, Kim J, Jeong DH, Lee Y-S, Jhang SH, Chung HJ, Cho E, Yu J-H, Jun B-H, Jung S. SERS-Based Flavonoid Detection Using Ethylenediamine-β-Cyclodextrin as a Capturing Ligand. Nanomaterials. 2017; 7(1):8.Chicago/Turabian Style
Choi, Jae M.; Hahm, Eunil; Park, Kyeonghui; Jeong, Daham; Rho, Won-Yeop; Kim, Jaehi; Jeong, Dae H.; Lee, Yoon-Sik; Jhang, Sung H.; Chung, Hyun J.; Cho, Eunae; Yu, Jae-Hyuk; Jun, Bong-Hyun; Jung, Seunho. 2017. "SERS-Based Flavonoid Detection Using Ethylenediamine-β-Cyclodextrin as a Capturing Ligand." Nanomaterials 7, no. 1: 8.
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