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Nanomaterials 2016, 6(1), 11; doi:10.3390/nano6010011

Repair of the Orbital Wall Fractures in Rabbit Animal Model Using Nanostructured Hydroxyapatite-Based Implant

1
Ophthalmology Department, “Carol Davila” University of Medicine and Pharmacy, 050474 Bucharest, Romania
2
National R & D Institute for Non-ferrous and Rare Metals, 077145 Ilfov, Romania
3
Pathology Department, “Carol Davila” University of Medicine and Pharmacy, 050474 Bucharest, Romania
4
Anatomy Department, “Carol Davila” University of Medicine and Pharmacy, 050474 Bucharest, Romania
5
Institute of Cellular Biology and Pathology “Nicolae Simionescu”, 050568 Bucharest, Romania
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Academic Editor: Andrea Danani
Received: 7 December 2015 / Revised: 21 December 2015 / Accepted: 29 December 2015 / Published: 7 January 2016
(This article belongs to the Special Issue Nanoparticles Assisted Drug Delivery)
View Full-Text   |   Download PDF [2611 KB, uploaded 7 January 2016]   |  

Abstract

Cellular uptake and cytotoxicity of nanostructured hydroxyapatite (nanoHAp) are dependent on its physical parameters. Therefore, an understanding of both surface chemistry and morphology of nanoHAp is needed in order to be able to anticipate its in vivo behavior. The aim of this paper is to characterize an engineered nanoHAp in terms of physico-chemical properties, biocompatibility, and its capability to reconstitute the orbital wall fractures in rabbits. NanoHAp was synthesized using a high pressure hydrothermal method and characterized by physico-chemical, structural, morphological, and optical techniques. X-ray diffraction revealed HAp crystallites of 21 nm, while Scanning Electron Microscopy (SEM) images showed spherical shapes of HAp powder. Mean particle size of HAp measured by DLS technique was 146.3 nm. Biocompatibility was estimated by the effect of HAp powder on the adhesion and proliferation of mesenchymal stem cells (MSC) in culture. The results showed that cell proliferation on powder-coated slides was between 73.4% and 98.3% of control cells (cells grown in normal culture conditions). Computed tomography analysis of the preformed nanoHAp implanted in orbital wall fractures, performed at one and two months postoperative, demonstrated the integration of the implants in the bones. In conclusion, our engineered nanoHAp is stable, biocompatible, and may be safely considered for reconstruction of orbital wall fractures. View Full-Text
Keywords: nanostructured HAp; mesenchymal stem cells; orbital wall fracture; implant nanostructured HAp; mesenchymal stem cells; orbital wall fracture; implant
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Gradinaru, S.; Popescu, L.M.; Piticescu, R.M.; Zurac, S.; Ciuluvica, R.; Burlacu, A.; Tutuianu, R.; Valsan, S.-N.; Motoc, A.M.; Voinea, L.M. Repair of the Orbital Wall Fractures in Rabbit Animal Model Using Nanostructured Hydroxyapatite-Based Implant. Nanomaterials 2016, 6, 11.

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