The Influence of Ozonated Olive Oil-Loaded and Copper-Doped Nanohydroxyapatites on Planktonic Forms of Microorganisms
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of Nanocrystalline Hydroxyapatite
2.2. Characterisation
2.3. Spectrophotometric Examination
- Growth control (1 mL suspension of microorganisms);
- Sterility control (1 mL medium);
- Compound control (1 mL medium and 0.1% and 1% concentration);
- Test sample (1 mL of microorganism suspension and analyzed compound at a concentration of 0.1% and 1%).
2.4. Determining the Value of the Colony-Forming Units, CFU/mL
2.5. Determining the Value of the Minimal Inhibitory Concentration, MIC
2.6. Scanning Electron Microscopy
2.7. Statistical Methods
3. Results
3.1. Structural Analysis
3.2. Infrared Spectra
3.3. Micro-Raman Spectra
3.4. EDS Analysis
3.5. Spectrophotometric Indication
3.6. Determining the Value of the Minimal Inhibitory Concentration
3.7. Determining the Value of Colony-Forming Units, CFU/mL
3.8. Scanning Electron Microscopy
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Species | MIC (µg/ mL) | |||
---|---|---|---|---|
I | II | III | IV | |
Candida albicans | >5000 | 5000 | 5000 | 5000 |
Streptococcus mutans | >5000 | >5000 | 5000 | 5000 |
Lactobacillus rhamnosus | >5000 | >5000 | >5000 | >5000 |
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Zakrzewski, W.; Dobrzynski, M.; Nowicka, J.; Pajaczkowska, M.; Szymonowicz, M.; Targonska, S.; Sobierajska, P.; Wiglusz, K.; Dobrzynski, W.; Lubojanski, A.; et al. The Influence of Ozonated Olive Oil-Loaded and Copper-Doped Nanohydroxyapatites on Planktonic Forms of Microorganisms. Nanomaterials 2020, 10, 1997. https://doi.org/10.3390/nano10101997
Zakrzewski W, Dobrzynski M, Nowicka J, Pajaczkowska M, Szymonowicz M, Targonska S, Sobierajska P, Wiglusz K, Dobrzynski W, Lubojanski A, et al. The Influence of Ozonated Olive Oil-Loaded and Copper-Doped Nanohydroxyapatites on Planktonic Forms of Microorganisms. Nanomaterials. 2020; 10(10):1997. https://doi.org/10.3390/nano10101997
Chicago/Turabian StyleZakrzewski, Wojciech, Maciej Dobrzynski, Joanna Nowicka, Magdalena Pajaczkowska, Maria Szymonowicz, Sara Targonska, Paulina Sobierajska, Katarzyna Wiglusz, Wojciech Dobrzynski, Adam Lubojanski, and et al. 2020. "The Influence of Ozonated Olive Oil-Loaded and Copper-Doped Nanohydroxyapatites on Planktonic Forms of Microorganisms" Nanomaterials 10, no. 10: 1997. https://doi.org/10.3390/nano10101997