Next Article in Journal
Role of Gut Microbiota in Rheumatoid Arthritis
Next Article in Special Issue
Myopathology of Adult and Paediatric Mitochondrial Diseases
Previous Article in Journal
Minimal Residual Disease in Acute Myeloid Leukemia: Still a Work in Progress?
Previous Article in Special Issue
Use of the Ketogenic Diet to Treat Intractable Epilepsy in Mitochondrial Disorders
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
J. Clin. Med. 2017, 6(6), 58; doi:10.3390/jcm6060058

An Effective, Versatile, and Inexpensive Device for Oxygen Uptake Measurement

1
INSERM UMR 1141, Hôpital Robert Debré, 75019 Paris, France
2
Faculté de Médecine Denis Diderot, Université Paris Diderot—Paris 7, Site Robert Debré, 75013 Paris, France
3
MITOLOGICS S.A.S. Hôpital Robert Debré, 48 Bd Sérurier, 75019 Paris, France
*
Author to whom correspondence should be addressed.
Academic Editor: Iain P. Hargreaves
Received: 3 April 2017 / Revised: 19 May 2017 / Accepted: 6 June 2017 / Published: 8 June 2017
View Full-Text   |   Download PDF [2058 KB, uploaded 8 June 2017]   |  

Abstract

In the last ten years, the use of fluorescent probes developed to measure oxygen has resulted in several marketed devices, some unreasonably expensive and with little flexibility. We have explored the use of the effective, versatile, and inexpensive Redflash technology to determine oxygen uptake by a number of different biological samples using various layouts. This technology relies on the use of an optic fiber equipped at its tip with a membrane coated with a fluorescent dye (www.pyro-science.com). This oxygen-sensitive dye uses red light excitation and lifetime detection in the near infrared. So far, the use of this technology has mostly been used to determine oxygen concentration in open spaces for environmental studies, especially in aquatic media. The oxygen uptake determined by the device can be easily assessed in small volumes of respiration medium and combined with the measurement of additional parameters, such as lactate excretion by intact cells or the membrane potential of purified mitochondria. We conclude that the performance of by this technology should make it a first choice in the context of both fundamental studies and investigations for respiratory chain deficiencies in human samples. View Full-Text
Keywords: respiration assay; oxygen uptake; glycolysis; mitochondriopathy respiration assay; oxygen uptake; glycolysis; mitochondriopathy
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Bénit, P.; Chrétien, D.; Porceddu, M.; Yanicostas, C.; Rak, M.; Rustin, P. An Effective, Versatile, and Inexpensive Device for Oxygen Uptake Measurement. J. Clin. Med. 2017, 6, 58.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
J. Clin. Med. EISSN 2077-0383 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top