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J. Clin. Med. 2017, 6(12), 115; doi:10.3390/jcm6120115

Adipose Derived Stem Cells for Corneal Wound Healing after Laser Induced Corneal Lesions in Mice

1
Department of Ophthalmology, Azienda Ospedaliero Universitaria Santa Maria della Misericordia, Udine 33100, Italy
2
Department of Pathology, University of Udine, Azienda Ospedaliero Universitaria Santa Maria della Misericordia, Udine 33100, Italy
3
Department of Pharmacobiology, University of Calabria, Cosenza 87036, Italy
4
Instituto Universitario Fernández-Vega, Fundación de Investigación Oftalmológica, University of Oviedo, Oviedo 33006, Spain
5
Department of Plastic Surgery, University of Udine, Azienda Ospedaliero Universitaria Santa Maria della Misericordia, Udine 33100, Italy
*
Author to whom correspondence should be addressed.
Received: 9 October 2017 / Revised: 24 November 2017 / Accepted: 30 November 2017 / Published: 5 December 2017
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Abstract

The aim of our study was to assess the clinical effectiveness of topical adipose derived stem cell (ADSC) treatment in laser induced corneal wounds in mice by comparing epithelial repair, inflammation, and histological analysis between treatment arms. Corneal lesions were performed on both eyes of 40 mice by laser induced photorefractive keratectomy. All eyes were treated with topical azythromycin bid for three days. Mice were divided in three treatment groups (n = 20), which included: control, stem cells and basic serum; which received topical treatment three times daily for five consecutive days. Biomicroscope assessments and digital imaging were performed by two masked graders at 30, 54, 78, 100, and 172 h to analyze extent of fluorescein positive epithelial defect, corneal inflammation, etc. Immunohistochemical techniques were used in fixed eyes to assess corneal repair markers Ki67, α Smooth Muscle Actin (α-SMA) and E-Cadherin. The fluorescein positive corneal lesion areas were significantly smaller in the stem cells group on days 1 (p < 0.05), 2 (p < 0.02) and 3. The stem cell treated group had slightly better and faster re-epithelization than the serum treated group in the initial phases. Comparative histological data showed signs of earlier and better corneal repair in epithelium and stromal layers in stem cell treated eyes, which showed more epithelial layers and enhanced wound healing performance of Ki67, E-Cadherin, and α-SMA. Our study shows the potential clinical and histological advantages in the topical ADSC treatment for corneal lesions in mice. View Full-Text
Keywords: adipose derived stem cells; corneal wound healing; stem cell therapy; lipoaspirate; corneal re-epithelization adipose derived stem cells; corneal wound healing; stem cell therapy; lipoaspirate; corneal re-epithelization
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MDPI and ACS Style

Zeppieri, M.; Salvetat, M.L.; Beltrami, A.; Cesselli, D.; Russo, R.; Alcalde, I.; Merayo-Lloves, J.; Brusini, P.; Parodi, P.C. Adipose Derived Stem Cells for Corneal Wound Healing after Laser Induced Corneal Lesions in Mice. J. Clin. Med. 2017, 6, 115.

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