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Membranes 2014, 4(4), 764-777; doi:10.3390/membranes4040764

Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell Membranes

1
Laboratory of Quality in Marine Products, Graduate School of Bioresources, Mie University, 1577 Kurima Machiya-cho, Tsu, Mie 514-8507, Japan
2
Toray Research Centre, Inc., 1111 Tebiro, Kamakura, Kanagawa 248-8555, Japan
*
Author to whom correspondence should be addressed.
Received: 25 August 2014 / Revised: 13 October 2014 / Accepted: 28 October 2014 / Published: 13 November 2014
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Abstract

We isolated a high-purity carp glycophorin from carp erythrocyte membranes and prepared the oligosaccharide fraction from glycophorin by β-elimination [1]. The oligosaccharide fraction was separated into two components (P-1 and P-2) using a Glyco-Pak DEAE column. These O-linked oligosaccharides (P-1 and P-2) were composed of glucose, galactose, fucose, N-acetylgalactosamine and N-glycolylneuraminic acid (NeuGc). The P-1 and P-2 contained one and two NeuGc residues, respectively, and the P-1 exhibited bacteriostatic activity [1]. Using NMR and GC-MS, we determined that the structure of the bacteriostatic P-1 was NeuGcα2→6 (Fucα1→4) (Glcα1→3) Galβ1→4GalNAc-ol. This O-linked oligosaccharide was unique for a vertebrate with respect to the hexosamine and hexose linkages and its non-chain structure. View Full-Text
Keywords: fish; red blood cell membranes; erythrocyte; glycophorin; oligosaccharide; sialic acid; N-glycolylneuraminic acid fish; red blood cell membranes; erythrocyte; glycophorin; oligosaccharide; sialic acid; N-glycolylneuraminic acid
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Aoki, T.; Chimura, K.; Sugiura, H.; Mizuno, Y. Structure of a Sialo-Oligosaccharide from Glycophorin in Carp Red Blood Cell Membranes. Membranes 2014, 4, 764-777.

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