Membranes 2013, 3(3), 169-181; doi:10.3390/membranes3030169
Article

Affinity Separation of Lectins Using Porous Membranes Immobilized with Glycopolymer Brushes Containing Mannose or N-Acetyl-D-Glucosamine

1email, 1email, 2email, 1email and 1,* email
Received: 16 June 2013; in revised form: 19 July 2013 / Accepted: 23 July 2013 / Published: 30 July 2013
View Full-Text   |   Download PDF [2102 KB, uploaded 30 July 2013]
Abstract: Porous membranes with glycopolymer brushes were prepared as biomaterials for affinity separation. Glycopolymer brushes contained acrylic acid and D-mannose or N-acetyl-D-glucosamine, and were formed on substrates by surface-initiated atom transfer radical polymerization. The presence of glycopolymer brush was confirmed by X-ray photoelectron spectroscopy, contact angle, and ellipsometry measurements. The interaction between lectin and the glycopolymer immobilized on glass slides was confirmed using fluorescent-labeled proteins. Glycopolymer-immobilized surfaces exhibited specific adsorption of the corresponding lectin, compared with bovine serum albumin. Lectins were continuously rejected by the glycopolymer-immobilized membranes. When the protein solution was permeated through the glycopolymer-immobilized membrane, bovine serum albumin was not adsorbed on the membrane surface. In contrast, concanavalin A and wheat germ agglutinin were rejected by membranes incorporating D-mannose or N-acetyl-D-glucosamine, respectively. The amounts of adsorbed concanavalin A and wheat germ agglutinin was increased five- and two-fold that of adsorbed bovine serum albumin, respectively.
Keywords: glycopolymer; polymer brush; atom transfer radical polymerization; lectin; affinity separation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Ogata, Y.; Seto, H.; Murakami, T.; Hoshino, Y.; Miura, Y. Affinity Separation of Lectins Using Porous Membranes Immobilized with Glycopolymer Brushes Containing Mannose or N-Acetyl-D-Glucosamine. Membranes 2013, 3, 169-181.

AMA Style

Ogata Y, Seto H, Murakami T, Hoshino Y, Miura Y. Affinity Separation of Lectins Using Porous Membranes Immobilized with Glycopolymer Brushes Containing Mannose or N-Acetyl-D-Glucosamine. Membranes. 2013; 3(3):169-181.

Chicago/Turabian Style

Ogata, Yutaro; Seto, Hirokazu; Murakami, Tatsuya; Hoshino, Yu; Miura, Yoshiko. 2013. "Affinity Separation of Lectins Using Porous Membranes Immobilized with Glycopolymer Brushes Containing Mannose or N-Acetyl-D-Glucosamine." Membranes 3, no. 3: 169-181.


Membranes EISSN 2077-0375 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert