Next Article in Journal / Special Issue
Berry Fruit Consumption and Metabolic Syndrome
Previous Article in Journal
A Review of Antioxidant Peptides Derived from Meat Muscle and By-Products
Previous Article in Special Issue
Cranberries and Cancer: An Update of Preclinical Studies Evaluating the Cancer Inhibitory Potential of Cranberry and Cranberry Derived Constituents
Article Menu

Export Article

Open AccessArticle
Antioxidants 2016, 5(4), 33; doi:10.3390/antiox5040033

Tart Cherry Extracts Reduce Inflammatory and Oxidative Stress Signaling in Microglial Cells

USDA-ARS, Human Nutrition Research Center on Aging, Tufts University, 711 Washington Street, Boston, MA 02111, USA
*
Author to whom correspondence should be addressed.
Academic Editor: Dorothy Klimis-Zacas
Received: 7 July 2016 / Revised: 2 September 2016 / Accepted: 9 September 2016 / Published: 22 September 2016
(This article belongs to the Special Issue Berry Antioxidants in Health and Disease)
View Full-Text   |   Download PDF [1556 KB, uploaded 22 September 2016]   |  

Abstract

Tart cherries contain an array of polyphenols that can decrease inflammation and oxidative stress (OS), which contribute to cognitive declines seen in aging populations. Previous studies have shown that polyphenols from dark-colored fruits can reduce stress-mediated signaling in BV-2 mouse microglial cells, leading to decreases in nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression. Thus, the present study sought to determine if tart cherries—which improved cognitive behavior in aged rats—would be efficacious in reducing inflammatory and OS signaling in HAPI rat microglial cells. Cells were pretreated with different concentrations (0–1.0 mg/mL) of Montmorency tart cherry powder for 1–4 h, then treated with 0 or 100 ng/mL lipopolysaccharide (LPS) overnight. LPS application increased extracellular levels of NO and tumor necrosis factor-alpha (TNF-α), and intracellular levels of iNOS and cyclooxygenase-2 (COX-2). Pretreatment with tart cherry decreased levels of NO, TNF-α, and COX-2 in a dose- and time-dependent manner versus those without pretreatment; the optimal combination was between 0.125 and 0.25 mg/mL tart cherry for 2 h. Higher concentrations of tart cherry powder and longer exposure times negatively affected cell viability. Therefore, tart cherries (like other dark-colored fruits), may be effective in reducing inflammatory and OS-mediated signals. View Full-Text
Keywords: antioxidant; anti-inflammatory; polyphenols; anthocyanins; cytokines antioxidant; anti-inflammatory; polyphenols; anthocyanins; cytokines
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Shukitt-Hale, B.; Kelly, M.E.; Bielinski, D.F.; Fisher, D.R. Tart Cherry Extracts Reduce Inflammatory and Oxidative Stress Signaling in Microglial Cells. Antioxidants 2016, 5, 33.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Antioxidants EISSN 2076-3921 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top