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Antioxidants 2016, 5(1), 2; doi:10.3390/antiox5010002

Lactation Affects Isolated Mitochondria and Its Fatty Acid Composition but Has No Effect on Tissue Protein Oxidation, Lipid Peroxidation or DNA-Damage in Laboratory Mice

1
Research Institute of Wildlife Ecology, University of Veterinary Medicine, Savoyenstrasse 1, Vienna A-1160, Austria
2
Institute of Physiology, Pathophysiology and Biophysics, University of Veterinary Medicine, Veterinärplatz 1, Vienna A-1210, Austria
3
Institute of Veterinary Public Health, University of Veterinary Medicine, Veterinärplatz 1, Vienna A-1210, Austria
4
Institute of Pharmacology and Toxicology, University of Veterinary Medicine, Veterinärplatz 1, Vienna A-1210, Austria
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Adam Salmon
Received: 29 October 2015 / Revised: 29 December 2015 / Accepted: 31 December 2015 / Published: 11 January 2016
(This article belongs to the Special Issue Oxidative Stress and Aging: Past, Present and Future Concepts)
View Full-Text   |   Download PDF [471 KB, uploaded 11 January 2016]   |  

Abstract

Linking peak energy metabolism to lifespan and aging remains a major question especially when focusing on lactation in females. We studied, if and how lactation affects in vitro mitochondrial oxygen consumption and mitochondrial fatty acid composition. In addition, we assessed DNA damage, lipid peroxidation and protein carbonyls to extrapolate on oxidative stress in mothers. As model system we used C57BL/6NCrl mice and exposed lactating females to two ambient temperatures (15 °C and 22 °C) while they nursed their offspring until weaning. We found that state II and state IV respiration rates of liver mitochondria were significantly higher in the lactating animals than in non-lactating mice. Fatty acid composition of isolated liver and heart mitochondria differed between lactating and non-lactating mice with higher n-6, and lower n-3 polyunsaturated fatty acids in the lactating females. Surprisingly, lactation did not affect protein carbonyls, lipid peroxidation and DNA damage, nor did moderate cold exposure of 15 °C. We conclude that lactation increases rates of mitochondrial uncoupling and alters mitochondrial fatty acid composition thus supporting the “uncoupling to survive” hypothesis. Regarding oxidative stress, we found no impact of lactation and lower ambient temperature and contribute to growing evidence that there is no linear relationship between oxidative damage and lactation. View Full-Text
Keywords: lactation; protein oxidation; TBARS; isolated mitochondria; oxygen consumption; comet assay; mitochondrial fatty acid composition; C57BL/6NCrl lactation; protein oxidation; TBARS; isolated mitochondria; oxygen consumption; comet assay; mitochondrial fatty acid composition; C57BL/6NCrl
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Valencak, T.G.; Raith, J.; Staniek, K.; Gille, L.; Strasser, A. Lactation Affects Isolated Mitochondria and Its Fatty Acid Composition but Has No Effect on Tissue Protein Oxidation, Lipid Peroxidation or DNA-Damage in Laboratory Mice. Antioxidants 2016, 5, 2.

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