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Antioxidants 2014, 3(3), 544-558; doi:10.3390/antiox3030544
Article

Endogenous Phenolics in Hulls and Cotyledons of Mustard and Canola: A Comparative Study on Its Sinapates and Antioxidant Capacity

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Received: 23 April 2014; in revised form: 20 May 2014 / Accepted: 20 May 2014 / Published: 15 August 2014
(This article belongs to the Special Issue Antioxidants in Oils)
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Abstract: Endogenous sinapic acid (SA), sinapine (SP), sinapoyl glucose (SG) and canolol (CAN) of canola and mustard seeds are the potent antioxidants in various lipid-containing systems. The study investigated these phenolic antioxidants using different fractions of canola and mustard seeds. Phenolic compounds were extracted from whole seeds and their fractions: hulls and cotyledons, using 70% methanol by the ultrasonication method and quantified using HPLC-DAD. The major phenolics from both hulls and cotyledons extracts were SP, with small amounts of SG, and SA with a significant difference of phenolic contents between the two seed fractions. Cotyledons showed relatively high content of SP, SA, SG and total phenolics in comparison to hulls (p < 0.001). The concentration of SP in different fractions ranged from 1.15 ± 0.07 to 12.20 ± 1.16 mg/g and followed a decreasing trend- canola cotyledons > mustard cotyledons > mustard seeds > canola seeds > mustard hulls > canola hulls. UPLC-tandem Mass Spectrometry confirmed the presence of sinapates and its fragmentation in these extracts. Further, a high degree of correlation (r = 0.93) was noted between DPPH scavenging activity and total phenolic content.
Keywords: sinapic acid derivatives; sinapine; sinapoyl glucose; mustard; canola; hulls; cotyledons; antioxidant activity sinapic acid derivatives; sinapine; sinapoyl glucose; mustard; canola; hulls; cotyledons; antioxidant activity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Mayengbam, S.; Aachary, A.; Thiyam-Holländer, U. Endogenous Phenolics in Hulls and Cotyledons of Mustard and Canola: A Comparative Study on Its Sinapates and Antioxidant Capacity. Antioxidants 2014, 3, 544-558.

AMA Style

Mayengbam S, Aachary A, Thiyam-Holländer U. Endogenous Phenolics in Hulls and Cotyledons of Mustard and Canola: A Comparative Study on Its Sinapates and Antioxidant Capacity. Antioxidants. 2014; 3(3):544-558.

Chicago/Turabian Style

Mayengbam, Shyamchand; Aachary, Ayyappan; Thiyam-Holländer, Usha. 2014. "Endogenous Phenolics in Hulls and Cotyledons of Mustard and Canola: A Comparative Study on Its Sinapates and Antioxidant Capacity." Antioxidants 3, no. 3: 544-558.

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