Antioxidants 2014, 3(2), 455-471; doi:10.3390/antiox3020455
Article

Screening of Antioxidant Activity of Gentian Lutea Root and Its Application in Oil-in-Water Emulsions

1,2email, 1email, 3, 3email and 1,* email
Received: 25 January 2014; in revised form: 10 April 2014 / Accepted: 12 June 2014 / Published: 19 June 2014
(This article belongs to the Special Issue Antioxidants in Oils)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Gentiana Lutea root (G. Lutea) is a medicinal herb, traditionally used as a bitter tonic in gastrointestinal ailments for improving the digestive system. The active principles of G. Lutea were found to be secoiridoid bitter compounds as well as many other active compounds causing the pharmacological effects. No study to date has yet determined the potential of G. Lutea antioxidant activity on lipid oxidation. Thus, the aim of this study was to evaluate the effects of an extract of G. Lutea on lipid oxidation during storage of an emulsion. G. Lutea extracts showed excellent antioxidant activity measured by DPPH scavenging assay and Trolox equivalent antioxidant capacity (TEAC) assays. An amount of 0.5% w/w G. Lutea lyophilise was able to inhibit lipid oxidation throughout storage (p < 0.05). A mixture of G. Lutea with 0.1% (w/w) BSA showed a good synergic effect and better antioxidant activity in the emulsion. Quantitative results of HPLC showed that G. Lutea contained secoiridoid-glycosides (gentiopiocroside and sweroside) and post column analysis displayed radical scavenging activity of G. Lutea extract towards the ABTS radical. The results from this study highlight the potential of G. Lutea as a food ingredient in the design of healthier food commodities.
Keywords: Gentiana Lutea; lipid oxidation; antioxidant; HPLC
PDF Full-text Download PDF Full-Text [835 KB, uploaded 19 June 2014 09:24 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Azman, N.A.M.; Segovia, F.; Martínez-Farré, X.; Gil, E.; Almajano, M.P. Screening of Antioxidant Activity of Gentian Lutea Root and Its Application in Oil-in-Water Emulsions. Antioxidants 2014, 3, 455-471.

AMA Style

Azman NAM, Segovia F, Martínez-Farré X, Gil E, Almajano MP. Screening of Antioxidant Activity of Gentian Lutea Root and Its Application in Oil-in-Water Emulsions. Antioxidants. 2014; 3(2):455-471.

Chicago/Turabian Style

Azman, Nurul A.M.; Segovia, Francisco; Martínez-Farré, Xavier; Gil, Emilio; Almajano, María P. 2014. "Screening of Antioxidant Activity of Gentian Lutea Root and Its Application in Oil-in-Water Emulsions." Antioxidants 3, no. 2: 455-471.

Antioxidants EISSN 2076-3921 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert