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Antioxidants 2014, 3(2), 200-211; doi:10.3390/antiox3020200

Antioxidant Activity of Grapevine Leaf Extracts against Oxidative Stress Induced by Carbon Tetrachloride in Cerebral Cortex, Hippocampus and Cerebellum of Rats

1 Centro Universitário Metodista IPA, Coronel Joaquim Pedro Salgado 80, Porto Alegre 90420-060, Brazil 2 Universidade de Caxias do Sul, Francisco Getúlio Vargas 1130, Caxias do Sul 95070-560, Brazil
* Author to whom correspondence should be addressed.
Received: 26 December 2013 / Revised: 10 March 2014 / Accepted: 17 March 2014 / Published: 2 April 2014
(This article belongs to the Special Issue Oxidative Stress and Neurodegenerative Diseases)
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In recent years, it has become increasingly important to study the beneficial properties of derivatives of grapes and grapevine. The objective of this study was to determine the antioxidant activity of Vitis labrusca leaf extracts, comparing conventional and organic grapevines, in different brain areas of rats. We used male Wistar rats treated with grapevine leaf extracts for a period of 14 days, and on the 15th day, we administered in half of the rats, mineral oil and the other half, carbon tetrachloride (CCl4). The animals were euthanized by decapitation and the cerebral cortex, hippocampus and cerebellum were removed to assess oxidative stress parameters and the activity of antioxidant enzymes. Lipid peroxidation levels (TBARS) were unchanged. However, CCl4 induced oxidative damage to proteins in all tissues studied, and this injury was prevented by both extracts. Superoxide dismutase (SOD) activity was increased by CCl4 in the cerebral cortex and decreased in other tissues. However, CCl4 increased catalase (CAT) activity in the cerebellum and decreased it in the cerebral cortex. The SOD/CAT ratio was restored in the cerebellum by both extracts and only in the cerebral cortex by the organic extract.
Keywords: grapes; Vitis labrusca; total polyphenols; neuroprotection grapes; Vitis labrusca; total polyphenols; neuroprotection
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wohlenberg, M.; Almeida, D.; Bokowski, L.; Medeiros, N.; Agostini, F.; Funchal, C.; Dani, C. Antioxidant Activity of Grapevine Leaf Extracts against Oxidative Stress Induced by Carbon Tetrachloride in Cerebral Cortex, Hippocampus and Cerebellum of Rats. Antioxidants 2014, 3, 200-211.

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