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Microarrays 2014, 3(2), 103-136; doi:10.3390/microarrays3020103
Review

Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays

Received: 9 January 2014; in revised form: 28 March 2014 / Accepted: 9 April 2014 / Published: 17 April 2014
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Abstract: With the advent of new histopathological staining techniques (histochemistry, immunohistochemistry, in situ hybridization) and the discovery of thousands of new genes, mRNA, and proteins by molecular biology, the need grew for a technique to compare many different cells or tissues on one slide in a cost effective manner and with the possibility to easily track the identity of each specimen: the tissue array (TA). Basically, a TA consists of at least two different specimens per slide. TAs differ in the kind of specimens, the number of specimens installed, the dimension of the specimens, the arrangement of the specimens, the embedding medium, the technique to prepare the specimens to be installed, and the technique to construct the TA itself. A TA can be constructed by arranging the tissue specimens in a mold and subsequently pouring the mold with the embedding medium of choice. In contrast, preformed so-called recipient blocks consisting of the embedding medium of choice have punched, drilled, or poured holes of different diameters and distances in which the cells or tissue biopsies will be deployed manually, semi-automatically, or automatically. The costs of constructing a TA differ from a few to thousands of Euros depending on the technique/equipment used. Remarkably high quality TAs can be also achieved by low cost techniques.
Keywords: tissue microarrays; techniques; paraffin; OCT tissue microarrays; techniques; paraffin; OCT
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Vogel, U. Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays. Microarrays 2014, 3, 103-136.

AMA Style

Vogel U. Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays. Microarrays. 2014; 3(2):103-136.

Chicago/Turabian Style

Vogel, Ulrich. 2014. "Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays." Microarrays 3, no. 2: 103-136.

Microarrays EISSN 2076-3905 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert