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Appl. Sci. 2018, 8(6), 930; https://doi.org/10.3390/app8060930

Development of a Novel Scaffold of Chitosan, Type IV Collagen and Integrin α3β1 As Alternative Scaffold for Primary Culture of Podocytes

1
Immunology and Virology department, Faculty of Biological Science, Autonomous University of Nuevo Leon, Pedro de Alba s/n, San Nicolás de Los Garza, Nuevo León 66455, México
2
Immunology and Molecular Biology department, Academic Unit of Biological Science, Autonomous University of Zacatecas, Avenida de la Revolución Mexicana s/n, Guadalupe, Zacatecas 98600, México
3
Investigation and Postgraduate Unit, Faculty of Engineering, Autonomous University of Querétaro, Cerro de las Campanas s/n, Querétaro 76010, México
4
General Hospital “Luz Gonzalez Cosio”, Col. Ciudad Gobierno, Zacatecas 98160, México
*
Author to whom correspondence should be addressed.
Received: 24 April 2018 / Revised: 21 May 2018 / Accepted: 26 May 2018 / Published: 5 June 2018
(This article belongs to the Section Applied Biosciences and Bioengineering)
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Abstract

Loss of podocytes has been a main pathology present in renal diseases; the leak of these specialized cells increases the permeability of the glomerular basal membrane (GMB) and protein release affecting the glomeruli, the main structure of the kidney. The study of different physiopathology mechanism has been a challenge because of the short lifetime of podocytes in vitro. We obtained and characterized composites based on Chitosan (CTS), polyvinyl alcohol (PVA), type IV collagen and integrin α3β1 as a possible application in primary culture of podocytes. Podocytes were extracted from the urine of patients with Idiopathic Nephrotic Syndrome (INS). To evaluate biocompatibility, we assessed cell viability through the lactate dehydrogenase assay. Immunohistochemical staining was used to detect the expression of specific proteins from podocytes such as podocin, and podocalyxin and CD80, a marker of cellular stress. The results showed that our synthesis method promotes the copolymerization of the components in the scaffold. Due to its reactivity, the amine group of chitosan made links with type IV collagen and integrin α3β1. The swelling and degradation tests allowed us to select the material with the best mechanical properties for cellular culture. The expression of podocin and podocalyxin remains the same in the culture of podocytes on the scaffold; in contrast, CD80 expression increased. The viability of podocytes cultured on the CTS/PVA/type IV collagen/integrin α3β1 scaffold increased in comparison to the culture control. View Full-Text
Keywords: podocytes; half-life; chitosan; protein expression; scaffold podocytes; half-life; chitosan; protein expression; scaffold
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Zárate-Triviño, D.G.; Hernández-Martínez, S.P.; Bollain-y-Goytia-de-la-Rosa, J.J.; Franco-Molina, M.A.; Elizalde-Peña, E.A.; Hernández-Villegas, M.I.; Rangel-Ochoa, G.A.; Rodríguez-Padilla, C. Development of a Novel Scaffold of Chitosan, Type IV Collagen and Integrin α3β1 As Alternative Scaffold for Primary Culture of Podocytes. Appl. Sci. 2018, 8, 930.

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